Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish Burr, Sarah E. and Gobeli, Stefanie and Kuhnert, Peter and Goldschmidt-Clermont, Elinor and Frey, Joachim,, 60, 2753-2757 (2010), doi = https://doi.org/10.1099/ijs.0.011692-0, publicationName = Microbiology Society, issn = 1466-5026, abstract= Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835T and JF4413, produced non-diffusible green pigment following 4–5 days incubation on Luria–Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C16 : 1 ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1 ω7c. The DNA G+C content was 62.9 mol%. Sequence analysis of the 16S rRNA gene indicated 100 % sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the Pseudomonas chlororaphis subspecies: P. chlororaphis subsp. chlororaphis (99.84 %), P. chlororaphis subsp. aurantiaca (99.75 %) and P. chlororaphis subsp. aureofaciens (99.40 %). While DNA–DNA relatedness confirmed the placement of strains JF3835T and JF4413 as members of the species P. chlororaphis, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835T and JF4413 represent a novel subspecies of the species P. chlororaphis, for which the name Pseudomonas chlororaphis subsp. piscium subsp. nov. is proposed. The type strain is JF3835T (=NCIMB 14478T=DSM 21509T)., language=, type=