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f gen. nov., sp. nov., isolated from human clinical samples, emended description of the family and description of classis nov., ord. nov. and fam. nov. in the bacterial phylum

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  • Authors: Hélène Marchandin1,2 ,  Corinne Teyssier1 ,  Josiane Campos2 ,  Hélène Jean-Pierre1,2 ,  Frédéric Roger1 ,  Bernard Gay3 ,  Jean-Philippe Carlier4 , Estelle Jumas-Bilak1
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    • 1 1Université Montpellier 1, EA 3755, Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, 15, Avenue Charles Flahault, BP 14491, 34060 Montpellier Cedex 5, France 2 2Centre Hospitalier et Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France 3 3Université Montpellier 1 et Université Montpellier 2, UMR 5236-CPBS CNRS, Centre d'études d'agents pathogènes et Biotechnologies pour la Santé, Institut de Biologie, 4, Boulevard Henri IV, CS 69033, 34965 Montpellier Cedex 2, France 4 4Institut Pasteur, Centre National de Référence des Bactéries Anaérobies et du Botulisme, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
  • Hélène Marchandin
 [email protected]
  • First Published Online: 01 June 2010, International Journal of Systematic and Evolutionary Microbiology 60: 1271-1279, doi: 10.1099/ijs.0.013102-0
  • Subject: New Taxa - Firmicutes And Related Organisms
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gen. nov., sp. nov., isolated from human clinical samples, emended description of the family and description of classis nov., ord. nov. and fam. nov. in the bacterial phylum , Page 1 of 1

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  • Three strains of a hitherto unknown, Gram-negative, tiny, anaerobic coccus were collected from human clinical samples originating from skin and soft tissues. The three isolates displayed at least 99.9 % identity in their 16S rRNA gene sequences and more than 99.8 % identity in their gene sequences. The isolates were affiliated to the family , the coccobacillus being the most closely related species, but there was no more than 91.1 % identity in the 16S rRNA gene sequence between this species and the three isolates. Phylogeny based on the 16S rRNA gene confirmed that the three strains represent a novel and robust lineage within the current family . A similar genomic structure was demonstrated for the three isolates by PFGE-based analysis. Morphology and metabolic end products, as well as genotypic and phylogenetic data supported the proposal of the novel genus gen. nov., with the novel species sp. nov. [type strain ADV 07/08/06-B-1388 (=AIP 149.07=CIP 109806=DSM 21255=CCUG 56017) as type species]. Phylogenetic analyses based on the 16S rRNA gene sequences of members of the phylum and other phyla indicated that the family forms a robust lineage clearly separated from those of the classes ‘’, ‘’, and ‘’ in the phylum . Therefore, we propose that this family is a class-level taxon in the phylum , for which the name classis nov. is proposed, based on the Gram-negative type of cell wall of its members, with the type order ord. nov. In this order, a novel family, fam. nov., is proposed and description of the family is emended.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and gene sequences of gen. nov., sp. nov. ADV 07/08/06-B-1388 are FJ715930 and FJ715931, respectively.

  • PFGE migration of I-I-restricted DNAs, an ML tree based on 227 partial 16S rRNA gene sequences (1200 nt), showing relationships between members of the family , other members of the phylum and other selected phyla, and composition of datasets A and B are available with the online version of this paper.

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