RT Journal Article SR Electronic(1) A1 Madhaiyan, Munusamy A1 Poonguzhali, Selvaraj A1 Lee, Jung-Sook A1 Lee, Keun-Chul A1 Saravanan, Venkatakrishnan Sivaraj A1 Santhanakrishnan, PalaniYR 2010 T1 Microbacterium azadirachtae sp. nov., a plant-growth-promoting actinobacterium isolated from the rhizoplane of neem seedlings JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 7 SP 1687 OP 1692 DO https://doi.org/10.1099/ijs.0.015800-0 PB Microbiology Society, SN 1466-5034, AB Microbacterium strain AI-S262T was isolated from the rhizoplane of neem seedlings in the Botanical garden of Tamilnadu Agricultural University, Coimbatore, India, and subjected to phenotypic, chemotaxonomic and genetic characterization. Cells of this strain were Gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. Strain AI-S262T contained MK-12 and MK-13 as the main respiratory quinones, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as the predominant fatty acids, peptidoglycan-type B2β with glycolyl residues, and had a DNA G+C content of 69.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed 98.0–98.6 % pair-wise similarity with respect to close relatives in the genus Microbacterium. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 39%) between strain AI-S262T and its closest relatives. Data from DNA–DNA hybridization and phenotypic analyses supported the conclusion that strain AI-S262T represents a novel species in the genus Microbacterium, for which the name Microbacterium azadirachtae sp. nov. is proposed. The type strain is AI-S262T (=JCM 15681T =LMG 24772T =KCTC 19668T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.015800-0