1887

Abstract

A novel strictly anaerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped, Gram-negative bacterium (YIT 12066) was isolated from human faeces. The isolate was negative for catalase, oxidase, urease, hydrolysis of aesculin and gelatin, nitrate reduction and indole production. The major end products of glucose metabolism were succinate and acetate. The major cellular fatty acids (>10 %) were C, C 7, C 9, C 7 and C. The G+C content of the DNA was 40.3 mol%. 16S rRNA gene sequence analysis showed that strain YIT 12066 was most closely related to members of the family , with sequence similarity of 92–87 %. However, some phenotypic characteristics such as cellular morphology and the major fatty acid profile of strain YIT 12066 were markedly different from those of other members of the family . On the basis of both phylogenetic and phenotypic evidence, it is suggested that strain YIT 12066 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain of is YIT 12066 (=DSM 22608 =JCM 16073).

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2010-08-01
2024-03-28
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References

  1. Bryant M. P. 2005a; Genus I. Succinivibrio Bryant and Small 1956, 22AL. In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol. 2, part B pp 581–582 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
  2. Bryant M. P. 2005b; Genus IV. Succinimonas Bryant, Small, Bouma and Chu 1958, 21AL. In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol. 2, part B pp 586–587 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
  3. Chonan O., Matsumoto K., Watanuki M. 1995; Effect of galactooligosaccharides on calcium absorption and preventing bone loss in ovariectomized rats. Biosci Biotechnol Biochem 59:236–239 [CrossRef]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  5. Davis C. P., Cleven D., Brown J., Balish E. 1976; Anaerobiospirillum , a new genus of spiral-shaped bacteria. Int J Syst Bacteriol 26:498–504 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Saidi S. M., Liu S. L., Hashimoto Y., Yamamoto H., Yabuuchi E. 1990; Rapid procedure to determine the DNA base composition from small amounts of Gram-positive bacteria. FEMS Microbiol Lett 55:127–130
    [Google Scholar]
  7. Fadzilah M. N., Faizatul L. J., Hasibah M. S., Sam I. C., Bador M. K., Gan G. G., Abubakar S. 2009; Anaerobiospirillum succiniciproducens bacteraemia in a patient with acute lymphoblastic leukaemia. J Med Microbiol 58:142–143 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  9. Garrity G. M., Bell J. A., Lilburn T. 2005; Family incertae sedis II. Succinivibrionaceae Hippe, Hagelstein, Kramer, Swiderski and Stackebrandt 1999, 782VP . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. p–581 New York: Springer;
    [Google Scholar]
  10. Hippe H., Hagelstein A., Kramer I., Swiderski J., Stackebrandt E. 1999; Phylogenetic analysis of Formivibrio citricus , Propionivibrio dicarboxylicus , Anaerobiospirillum thomasii , Succinimonas amylolytica and Succinivibrio dextrinosolvens and proposal of Succinivibrionaceae fam. nov. Int J Syst Bacteriol 49:779–782 [CrossRef]
    [Google Scholar]
  11. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  12. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  13. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441 [CrossRef]
    [Google Scholar]
  14. Malnick H. 1997; Anaerobiospirillum thomasii sp. nov., an anaerobic spiral bacterium isolated from the feces of cats and dogs and from diarrheal feces of humans, and emendation of the genus Anaerobiospirillum . Int J Syst Bacteriol 47:381–384 [CrossRef]
    [Google Scholar]
  15. Malnick H. 2005; Genus II. Anaerobiospirillum Davis, Cleven, Brown and Balish 1976, 503AL emend. Malnick 1997 383 In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. pp 582–583 New York: Springer;
    [Google Scholar]
  16. Malnick H., Thomas M. E. M., Lotay H., Robbins M. 1983; Anaerobiospirillum species isolated from humans with diarrhoea. J Clin Pathol 36:1097–1101 [CrossRef]
    [Google Scholar]
  17. Malnick H., Williams K., Phil-Ebosie J., Levy A. S. 1990; Description of a medium for isolating Anaerobiospirillum spp., a possible cause of zoonotic disease, from diarrheal feces and blood of humans and use of the medium in a survey of human, canine, and feline feces. J Clin Microbiol 28:1380–1384
    [Google Scholar]
  18. Marcus L., Gove E. W., van der Walt M. L., Koornhof H. J., Malnick H., Kilian J. G. 1996; First reported African case of Anaerobiospirillum succiniciproducens septicemia. Eur J Clin Microbiol Infect Dis 15:741–744 [CrossRef]
    [Google Scholar]
  19. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  20. Moore L. V. H., Bourne D. M., Moore W. E. C. 1994; Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic Gram-negative bacilli. Int J Syst Bacteriol 44:338–347 [CrossRef]
    [Google Scholar]
  21. Morotomi M., Nagai F., Sakon H., Tanaka R. 2008; Dialister succinatiphilus sp. nov. and Barnesiella intestinihominis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 58:2716–2720 [CrossRef]
    [Google Scholar]
  22. Morotomi M., Nagai F., Sakon H., Tanaka R. 2009 Paraprevotella clara gen. nov., sp. nov., and Paraprevotella xylaniphila sp. nov., members of the family ‘ Prevotellaceae ’ isolated from human faeces Int J Syst Evol Microbiol 59:1895–1900 [CrossRef]
    [Google Scholar]
  23. Nagai F., Morotomi M., Sakon H., Tanaka R. 2009; Parasutterella excrementihominis gen. nov., sp. nov., a member of the family Alcaligenaceae isolated from human faeces. Int J Syst Evol Microbiol 59:1793–1797 [CrossRef]
    [Google Scholar]
  24. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  25. Pienaar C., Kruger A. J., Venter E. C., Pitout J. D. 2003; Anaerobiospirillum succiniciproducens bacteraemia. J Clin Pathol 56:316–318 [CrossRef]
    [Google Scholar]
  26. Rifkin G. D., Opdyke J. E. 1981; Anaerobiospirillum succiniciproducens septicemia. J Clin Microbiol 13:811–813
    [Google Scholar]
  27. Rossi M., Hänninen M. L., Revez J., Hannula M., Zanoni R. G. 2008; Occurrence and species level diagnostics of Campylobacter spp., enteric Helicobacter spp. and Anaerobiospirillum spp. in healthy and diarrheic dogs and cats. Vet Microbiol 129:304–314 [CrossRef]
    [Google Scholar]
  28. Rudensky B., Wachtel D., Yinnon A. M., Raveh D., Schlesinger Y. 2002; Anaerobiospirillum succiniciproducens bacteremia in a young child. Pediatr Infect Dis J 21:575–576 [CrossRef]
    [Google Scholar]
  29. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  30. Sakon H., Nagai F., Morotomi M., Tanaka R. 2008; Sutterella parvirubra sp. nov. and Megamonas funiformis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 58:970–975 [CrossRef]
    [Google Scholar]
  31. Scupham A. J. 2007; Succession in the intestinal microbiota of preadolescent turkeys. FEMS Microbiol Ecol 60:136–147 [CrossRef]
    [Google Scholar]
  32. Secchi C., Cantarelli V. V., Pereira F. de S., Wolf H. H., Brodt T. C., Amaro M. C., Inamine E. 2005; Fatal bacteremia due to Anaerobiospirillum succiniciproducens : first description in Brazil. Braz J Infect Dis 9:169–172 [CrossRef]
    [Google Scholar]
  33. Stackebrandt E. S. 2005; Genus III. Ruminobacter Stackebrandt and Hippe 1987, 179VP (Effective publication: Stackebrandt and Hippe; 1986, 205; In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 2 part B Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. pp 584–586 New York: Springer;
  34. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  35. Tee W., Korman T. M., Waters M. J., Macphee A., Jenney A., Joyce L., Dyall-Smith M. L. 1998; Three cases of Anaerobiospirillum succiniciproducens bacteremia confirmed by 16S rRNA gene sequencing. J Clin Microbiol 36:1209–1213
    [Google Scholar]
  36. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
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