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Volume 60, Issue 7

sp. nov., isolated from a tidal flat sediment Free

Abstract

A Gram-negative-staining, non-motile, non-spore-forming and strictly aerobic bacterial strain, SC35, was isolated from tidal flat sediment collected from the South Sea, Korea, and subjected to a taxonomic study using a polyphasic approach. The organism grew optimally at 20–30 °C and with 1–2 % (w/v) NaCl. Strain SC35 contained ubiquinone-8 as the predominant respiratory lipoquinone and C 9 as the major fatty acid. The DNA GC content was 48.5 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain SC35 formed a lineage within the genus (94.3–96.5 % sequence similarity), forming a distinct branch in a clade also containing NIBH P2K6 and SW-238. On the basis of phenotypic and phylogenetic data, strain SC35 (=KCTC 22503=JCM 16343) was placed in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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2010-07-01
2024-04-18
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References

  1. Bakermans C., Ayala-del-Río H. L., Ponder M. A., Vishnivetskaya T., Gilichinsky D., Thomashow M. F., Tiedje J. M. 2006 Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost. Int J Syst Evol Microbiol 56, 1285–1291 [CrossRef]
  2. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
     [Google Scholar]
  3. Bowman J. P., Cavanagh J., Austin J. J., Sanderson K. 1996; Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46:841–848 [CrossRef]
     [Google Scholar]
  4. Bowman J. P., Nichols D. S., McMeekin T. A. 1997; Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic sea ice. Syst Appl Microbiol 20:209–215 [CrossRef]
     [Google Scholar]
  5. Bozal N., Montes J., Tudela E., Guinea J. 2003; Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp.nov. and Psychrobacter fozii sp. nov. . Int J Syst Evol Microbiol 53:1093–1100 [CrossRef]
     [Google Scholar]
  6. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
     [Google Scholar]
  7. Chun J., Bae K. S., Moon E. Y., Jung S. O., Lee H. K., Kim S. J. 2000; Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50:1909–1913
     [Google Scholar]
  8. CLSI 2003 Performance Standards for Antimicrobial Disk Susceptibility Tests , 8th edn. Approved Standard M2–A8. Wayne, PA: Clinical Laboratory Standards Institute;
     [Google Scholar]
  9. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  10. Denner E. B. M., Mark B., Busse H.-J., Turkiewicz M., Lubitz W. 2001; Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease. Syst Appl Microbiol 24:44–53 [CrossRef]
     [Google Scholar]
  11. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  12. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
     [Google Scholar]
  13. González C. J., Santos J. A., García-López M. L., Otero A. 2000; Psychrobacters and related bacteria in freshwater fish. J Food Prot 63:315–321
     [Google Scholar]
  14. Heuchert A., Glöckner F. O., Amann R., Fischer U. 2004; Psychrobacter nivimaris sp. nov., a heterotrophic bacterium attached to organic particles isolated from the South Atlantic (Antarctica). Syst Appl Microbiol 27:399–406 [CrossRef]
     [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  16. Jung S.-Y., Lee M.-H., Oh T.-K., Park Y.-H., Yoon J.-H. 2005; Psychrobacter cibarius sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55:577–582 [CrossRef]
     [Google Scholar]
  17. Juni E., Heym G. A. 1986; Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of Gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36:388–391 [CrossRef]
     [Google Scholar]
  18. Kämpfer P., Albrecht A., Buczolits S., Busse H.-J. 2002; Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. Syst Appl Microbiol 25:31–36 [CrossRef]
     [Google Scholar]
  19. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178: 703
    [Google Scholar]
  20. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
     [Google Scholar]
  21. Maruyama A., Honda D., Yamamoto H., Kitamura K., Higashihara T. 2000; Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep-sea species Psychrobacter pacificensis sp. nov. Int J Syst Evol Microbiol 50:835–846 [CrossRef]
     [Google Scholar]
  22. MIDI 1999 Sherlock Microbial Identification System Operating Manual , version 3.0 Newark, DE: MIDI Inc;
     [Google Scholar]
  23. Richard C., Kiredjian M. 1995 Laboratory Methods for the Identification of Strictly Aerobic Gram - negative Bacilli Paris: Institut Pasteur;
     [Google Scholar]
  24. Romanenko L. A., Schumann P., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2002; Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments. Int J Syst Evol Microbiol 52:1291–1297 [CrossRef]
     [Google Scholar]
  25. Romanenko L. A., Lysenko A. M., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54:1741–1745 [CrossRef]
     [Google Scholar]
  26. Romanenko L. A., Tanlka N., Frolova G. M., Mikhailov V. V. 2009; Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan. Int J Syst Evol Microbiol 59:1480–1489 [CrossRef]
     [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  28. Shivaji S., Reddy G. S. N., Raghavan P. U. M., Sarita N. B., Deille D. 2004 Psychrobacter salsus sp. nov. and Psychrobacter adeliensis sp. nov. isolated from fast ice from Adelie Land, Antarctica. Syst Appl Microbiol 27, 628–635 [CrossRef]
  29. Shivaji S., Reddy G. S. N., Suresh K., Gupta P., Chintalapati S., Schumann P., Stackebrandt E., Matsumoto G. I. 2005; Psychrobacter vallis sp. nov. and Psychrobacter aquaticus sp. nov., from Antarctica. Int J Syst Evol Microbiol 55:757–762 [CrossRef]
     [Google Scholar]
  30. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
     [Google Scholar]
  31. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  32. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  33. Swofford D. L. 1998 Phylogenetic analysis using parsimony (paup), version 4 Sunderland, MA: Sinauer Associates;
     [Google Scholar]
  34. Vela A. I., Collins M. D., Latre M. V., Mateos A., Moreno M. A., Hutson R., Domínguez L., Fernández-Garayzábal J. F. 2003; Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int J Syst Evol Microbiol 53:415–419 [CrossRef]
     [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  36. Yamaguchi S., Yokoe M. 2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343 [CrossRef]
     [Google Scholar]
  37. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
     [Google Scholar]
  38. Yoon J.-H., Lee C.-H., Yeo S.-H., Oh T.-K. 2005a; Psychrobacter aquimaris sp. nov. and Psychrobacter namhaensis sp. nov. isolated from sea water of the South Sea in Korea. Int J Syst Evol Microbiol 55:1007–1013 [CrossRef]
     [Google Scholar]
  39. Yoon J.-H., Yeo S.-H., Oh T.-K., Park Y.-H. 2005b; Psychrobacter alimentarius sp. nov., isolated from squid jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55:171–176 [CrossRef]
     [Google Scholar]
  40. Yoon J. H., Lee C. H., Kang S. J., Oh T. K. 2005c; Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea. Int J Syst Evol Microbiol 55:1885–1890 [CrossRef]
     [Google Scholar]
  41. Yumoto I., Hirota K., Sogabe Y., Nodasaka Y., Yokota Y., Hoshino T. 2003; Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 53:1985–1989 [CrossRef]
     [Google Scholar]
  42. Yumoto I., Hirota K., Kimoto H., Nodasaka Y., Matsuyama H., Yoshimune K. 2009; Psychrobacter piscatorii sp. nov., psychrotolerant bacterium exhibiting high catalase activity isolated from oxidative environment. Int J Syst Evol Microbiol 60:205–208
     [Google Scholar]
  43. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
     [Google Scholar]
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Psychrobacter aestuarii sp. nov., isolated from a tidal flat sediment
Int J Syst Evol Microbiol 60, 1631 (2010); https://doi.org/10.1099/ijs.0.016782-0
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