1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 10

sp. nov., isolated from salted crab () in Thailand Free

Abstract

A Gram-positive, endospore-forming, rod-shaped bacterium, strain PD-A10, was isolated from salted crab () in Thailand and subjected to a taxonomic study. Phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus . The novel strain grew in medium with 0–14 % (w/v) NaCl, at 4–55 °C and at pH 4.5–9. The predominant quinone was a menaquinone with seven isoprene units (MK-7). The major fatty acids were anteiso-C and anteiso-C. Polar lipid analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol, glycolipid and unknown lipids. The DNA G+C content was 41.4 mol%. The 16S rRNA gene sequence similarities between strain PD-A10 and NBRC 15535, DSM 10, DSM 11031 and IFO 15718 were 99.5, 99.4, 99.4 and 99.2 %, respectively. Strain PD-A10 showed a low degree similarity of rep-PCR fingerprints and low DNA–DNA relatedness with the above-mentioned species. On the basis of the data gathered in this study, strain PD-A10 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PD-A10 (=BCC 22614=KCTC 13613).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.018879-0
2010-10-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/10/2364.html?itemId=/content/journal/ijsem/10.1099/ijs.0.018879-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press;
     [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
     [Google Scholar]
  3. Chokesajjawatee N., Zo Y. G., Colwell R. R. 2008; Determination of clonality and relatedness of Vibrio cholerae isolates by genomic fingerprinting, using long-range repetitive element sequence-based PCR. Appl Environ Microbiol 74:5392–5401 [CrossRef]
     [Google Scholar]
  4. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872174AL . In Bergey's Manual of Systematic Bacteriology vol 2 pp 1105–1139 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  5. Crisan E. V., Sands A. 1975; Microflora of four fermented fish sauces. Appl Microbiol 29:106–108
     [Google Scholar]
  6. Euzéby J. P. 2009; List of bacterial names with standing in nomenclature: a folder available on the Internet. [Last full update 25 August 2009] http://www.bacterio.net
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  9. Gevers D., Huys G., And Swings J. 2001; Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31–36 [CrossRef]
     [Google Scholar]
  10. Heyrman J., Balcaen A., Rodriguez-Diaz M., Logan N. A., Swings J., Vos P. D. 2003; Bacillus decolorationis sp. nov., isolated from biodeteriorated parts of the mural paintings at the Servilia tomb (Roman necropolis of Carmona, Spain) and the Saint-Catherine chapel (Castle Herberstein, Austria. Int J Syst Evol Microbiol 53:459–463 [CrossRef]
     [Google Scholar]
  11. Hucker G. L., Conn H. J. 1923; Method of Gram staining. N Y State Agric Exp Sta Tech Bull 93:3–37
     [Google Scholar]
  12. Kim H.-K., Kim G.-T., Kim D.-K., Choi W.-A., Park S.-H., Jeong Y.-K., Kong I.-S. 1997; Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA 38 originated from fermented fish. J Ferment Bioeng 84:307–312 [CrossRef]
     [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
     [Google Scholar]
  15. Kumar S., Tamura K., Jakobson I. B., Nei M. 2001; mega 2: Molecular evolution analysis software. Bioinformatics 17:1244–1245 [CrossRef]
     [Google Scholar]
  16. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J Gen Microbiol 130:1871–1882
     [Google Scholar]
  17. Nick G., Jussila M., Hoste B., Niemi R. M., Kaijalainen S., de Lajudie P., Gillis M., de Bruijn F. J., Lindström K. 1999; Rhizobia isolated from root nodules of tropical leguminous trees characterized using DNA-DNA dot-blot hybridization and rep-PCR genomic fingerprinting. Syst Appl Microbiol 22:287–299 [CrossRef]
     [Google Scholar]
  18. Noguchi H., Uchino M., Shida O., Takano K., Nakamura L. K., Komagata K. 2004; Bacillus vietnamensis sp. nov., a moderately halotolerant, aerobic, endospore-forming bacterium isolated from Vietnamese fish sauce. Int J Syst Evol Microbiol 54:2117–2120 [CrossRef]
     [Google Scholar]
  19. Phithakpol B., Varanyanond W., Reungmaneepaitoon S., Wood H. 1995 The Traditional Fermented Foods of Thailand Kuala Lumpur: ASEAN Food Handling Bureau Level;3
     [Google Scholar]
  20. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus . J Gen Microbiol 134:1847–1882
     [Google Scholar]
  21. Rademaker J. L. W., Hoste B., Louws F. J., Kersters K., Swings J., Vauterin L., Vauterin P., de Bruijn F. J. 2000; Comparison of AFLP and rep-PCR genomic fingerprinting with DNA-DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50:665–677 [CrossRef]
     [Google Scholar]
  22. Rhodes M. E. 1958; The cytology of Pseudomonas spp. as revealed by a silver-plating staining method. J Gen Microbiol 18:639–648 [CrossRef]
     [Google Scholar]
  23. Roberts M. S., Nakamura L. K., Cohan F. M. 1994; Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. Int J Syst Bacteriol 44:256–264 [CrossRef]
     [Google Scholar]
  24. Roberts M. S., Nakamura L. K., Cohan F. M. 1996; Bacillus vallismortis sp. nov., a close relative of Bacillus subtilis isolated from soil in Death Valley, California. Int J Syst Bacteriol 46:470–475 [CrossRef]
     [Google Scholar]
  25. Saisithi P., Kasemsarn R. O., Liston J., Dollar A. M. 1966; Microbiology and chemistry of fermented fish. J Food Sci 31:105–110 [CrossRef]
     [Google Scholar]
  26. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [CrossRef]
     [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  28. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE, USA: Microbial ID, Inc;
     [Google Scholar]
  29. Schallmey M., Singh A., Ward O. P. 2004; Developments in the use of Bacillus species for industrial production. Can J Microbiol 50:1–17 [CrossRef]
     [Google Scholar]
  30. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946 [CrossRef]
     [Google Scholar]
  31. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thaiminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
     [Google Scholar]
  32. Takagi H., Shida O., Kadowaki K., Komagata K., Udaka S. 1993; Characterization of Bacillus brevis with descriptions of Bacillus migulanus sp.nov., Bacilluschoshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.. Int J Syst Bacteriol 43:221–231 [CrossRef]
     [Google Scholar]
  33. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak K., Jeanmougin F., Higgins D. G. 1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignments aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
     [Google Scholar]
  35. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
     [Google Scholar]
  36. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
     [Google Scholar]
  37. Versalovic M., Schneider M., de Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
     [Google Scholar]
  38. Yoon J.-H., Kang S.-S., Lee K.-C., Kho Y. H., Choi S. H., Kang K. H., Park Y.-H. 2001; Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood. Int J Syst Evol Microbiol 51:1087–1092 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.018879-0
Loading
Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand
Int J Syst Evol Microbiol 60, 2364 (2010); https://doi.org/10.1099/ijs.0.018879-0
/content/journal/ijsem/10.1099/ijs.0.018879-0
/content/journal/ijsem/10.1099/ijs.0.018879-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error