RT Journal Article SR Electronic(1) A1 Sumpavapol, Punnanee A1 Tongyonk, Linna A1 Tanasupawat, Somboon A1 Chokesajjawatee, Nipa A1 Luxananil, Plearnpis A1 Visessanguan, WonnopYR 2010 T1 Bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in Thailand JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 10 SP 2364 OP 2370 DO https://doi.org/10.1099/ijs.0.018879-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, endospore-forming, rod-shaped bacterium, strain PD-A10T, was isolated from salted crab (poo-khem) in Thailand and subjected to a taxonomic study. Phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus Bacillus. The novel strain grew in medium with 0–14 % (w/v) NaCl, at 4–55 °C and at pH 4.5–9. The predominant quinone was a menaquinone with seven isoprene units (MK-7). The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. Polar lipid analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol, glycolipid and unknown lipids. The DNA G+C content was 41.4 mol%. The 16S rRNA gene sequence similarities between strain PD-A10T and Bacillus amyloliquefaciens NBRC 15535T, Bacillus subtilis DSM 10T, Bacillus vallismortis DSM 11031T and Bacillus mojavensis IFO 15718T were 99.5, 99.4, 99.4 and 99.2 %, respectively. Strain PD-A10T showed a low degree similarity of rep-PCR fingerprints and low DNA–DNA relatedness with the above-mentioned species. On the basis of the data gathered in this study, strain PD-A10T should be classified as representing a novel species of the genus Bacillus, for which the name Bacillus siamensis sp. nov. is proposed. The type strain is PD-A10T (=BCC 22614T=KCTC 13613T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.018879-0