@article{mbs:/content/journal/ijsem/10.1099/ijs.0.019273-0, author = "Tian, Jianqing and Wang, Yanfen and Dong, Xiuzhu", title = "Methanoculleus hydrogenitrophicus sp. nov., a methanogenic archaeon isolated from wetland soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "9", pages = "2165-2169", doi = "https://doi.org/10.1099/ijs.0.019273-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.019273-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "An obligately anaerobic, methanogenic archaeon, strain HCT, was isolated from soil of the Zoige wetland on the Tibetan plateau, China. The strain was isolated through construction of an artificial butyrate-degrading consortium in co-culture with a syntrophic bacterium, ‘Syntrophomonas erecta subsp. sporosyntropha’ JCM 13344. Cells of strain HCT were irregular coccoids, 0.8–2 μm in diameter, that occurred singly and utilized only H2/CO2 for growth and methane production. Growth occurred at 18–45 °C (optimum around 37 °C). The pH for growth was 5.0–8.5 (optimal growth around pH 6.6). The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence analysis indicated that strain HCT was affiliated to the genus Methanoculleus, with sequence similarities of 94.8–97.2 % to existing members. However, strain HCT was distinguished from described Methanoculleus species by not using formate for growth or methane formation and not requiring acetate as a growth factor. On the basis of phylogenetic analysis and phenotypic characteristics, the novel species Methanoculleus hydrogenitrophicus sp. nov. is proposed, with strain HCT (=CGMCC 1.5146T =JCM 16311T) as the type strain.", }