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f sp. nov., isolated from root nodules of

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  • Authors: Rong Juan Zhang1 ,  Bao Chao Hou1 ,  En Tao Wang2 ,  Ying Li Jr1 ,  Xiao Xia Zhang3 , Wen Xin Chen1
    • VIEW AFFILIATIONS
    • 1 1State Key Laboratories for Agro-Biotechnology, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China 2 2Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D. F., Mexico 3 3Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
  • WenXin Chen
 [email protected]
  • First Published Online: 01 March 2011, International Journal of Systematic and Evolutionary Microbiology 61: 512-517, doi: 10.1099/ijs.0.020156-0
  • Subject: New Taxa - Proteobacteria
  • Cover date:
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  • Four rhizobial strains, designated CCBAU 85046, CCBAU 85051, CCBAU 85048 and CCBAU 85049, isolated from root nodules of grown in Tibet, China, were previously defined, using amplified 16S rRNA gene restriction analysis, as a novel group within the genus . To clarify their taxonomic position, these strains were further analysed and compared with reference strains of related bacteria using a polyphasic approach. The 16S rRNA gene analysis showed that the four isolates formed a distinct phylogenetic lineage in the genus . The isolates showed highest sequence similarity (97.8 %) to CCBAU 71042. Phenotypic and physiological tests, DNA–DNA hybridization, phylogenetic analyses of housekeeping genes , and and fatty acid profiles also indicated that these four strains constitute a novel group distinct from recognized species of the genus . Based on this evidence, strains CCBAU 85046, CCBAU 85051, CCBAU 85048 and CCBAU 85049 represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CCBAU 85046 (=LMG 25225 =HAMBI 3066) and its DNA G+C content is 59.52 mol% ( ). Strain CCBAU 85046 could form effective nodules on plant species and but not on its host of origin .

  • The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA, 16S–23S rRNA intergenic spacer, , and gene sequences of strain CCBAU 85046 are EU256434, EU288745, EU288670, EU288696 and DQ985380, respectively. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains CCBAU 85051, CCBAU 85048 and CCBAU 85049 are GU938296, GU938297 and GU938298, respectively.

  • Four supplementary figures and one supplementary table are available with the online version of this paper.

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2019-02-22
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