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f sp. nov., associated with mortality in the Chinese mitten crab,

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  • Authors: Wen Wang1 ,  Wei Gu1 ,  Gail E. Gasparich2 ,  Keran Bi1,3 ,  Jiangtao Ou1 ,  Qingguo Meng1 ,  Tingming Liang1 ,  Qi Feng1 ,  Jianqiong Zhang4 , Ying Zhang4
    • VIEW AFFILIATIONS
    • 1 1Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, PR China 2 2Department of Biological Sciences, Towson University, Towson, MD 21252, USA 3 3Jiangsu Key Laboratory for Marine Biotechnology, Huaihai Institute of Technology, Lian, Yungang 222005, PR China 4 4Medical School of Southeast University, Nanjing 210009, PR China
  • Wen Wang [email protected]
  • First Published Online: 01 April 2011, International Journal of Systematic and Evolutionary Microbiology 61: 703-708, doi: 10.1099/ijs.0.020529-0
  • Subject: New Taxa - Other Bacteria
  • Cover date:
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  • A motile bacterium, designated strain TDA-040725-5, was isolated from the haemolymph of a Chinese mitten crab, , with tremor disease. Based on 16S rRNA gene sequence analysis, the strain was phylogenetically distinct from other spiroplasmas but was closely related to ATCC 29335. Cells of strain TDA-040725-5 were variable in length and shape, helical and motile, as determined by phase-contrast light microscopy. Examination by electron microscopy revealed wall-less cells delimited by a single membrane. The strain grew in M1D or R-2 liquid media at 20–40 °C, with optimum growth at 30 °C. Doubling time at the optimal temperature was 24 h. The strain catabolized glucose and hydrolysed arginine but did not hydrolyse urea. The DNA G+C content was 29.7±1 mol%. The genome size was ~1.4–1.6 Mbp. Serological analysis, performed using the deformation test, did not reveal any reciprocal titres ≥320, indicating that strain TDA-040725-5 had minimal cross-reactivity to strains of recognized species of the genus . Based on this evidence, strain TDA-040725-5 ( = CCTCC M 207170  = DSM 21848) represents a novel species of the genus , for which the name sp. nov. is proposed, belonging to the novel serological group XLIII.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence of strain TDA-040725-5 is DQ917753.

  • Abbreviations:
    CCU colour-change unit
    DF deformation
    MP maximum-parsimony
    ML maximum-likelihood
    TD tremor disease
    TDA tremor disease agent
    TEM transmission electron microscopy
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