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- Volume 61, Issue 4
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f Spiroplasma eriocheiris sp. nov., associated with mortality in the Chinese mitten crab, Eriocheir sinensis
- Authors: Wen Wang1 , Wei Gu1 , Gail E. Gasparich2 , Keran Bi1,3 , Jiangtao Ou1 , Qingguo Meng1 , Tingming Liang1 , Qi Feng1 , Jianqiong Zhang4 , Ying Zhang4
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- VIEW AFFILIATIONS
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1 1Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, PR China 2 2Department of Biological Sciences, Towson University, Towson, MD 21252, USA 3 3Jiangsu Key Laboratory for Marine Biotechnology, Huaihai Institute of Technology, Lian, Yungang 222005, PR China 4 4Medical School of Southeast University, Nanjing 210009, PR China
- Correspondence Wen Wang [email protected]
- First Published Online: 01 April 2011, International Journal of Systematic and Evolutionary Microbiology 61: 703-708, doi: 10.1099/ijs.0.020529-0
- Subject: New Taxa - Other Bacteria
- Cover date:




Spiroplasma eriocheiris sp. nov., associated with mortality in the Chinese mitten crab, Eriocheir sinensis, Page 1 of 1
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A motile bacterium, designated strain TDA-040725-5T, was isolated from the haemolymph of a Chinese mitten crab, Eriocheir sinensis, with tremor disease. Based on 16S rRNA gene sequence analysis, the strain was phylogenetically distinct from other spiroplasmas but was closely related to Spiroplasma mirum ATCC 29335T. Cells of strain TDA-040725-5T were variable in length and shape, helical and motile, as determined by phase-contrast light microscopy. Examination by electron microscopy revealed wall-less cells delimited by a single membrane. The strain grew in M1D or R-2 liquid media at 20–40 °C, with optimum growth at 30 °C. Doubling time at the optimal temperature was 24 h. The strain catabolized glucose and hydrolysed arginine but did not hydrolyse urea. The DNA G+C content was 29.7±1 mol%. The genome size was ~1.4–1.6 Mbp. Serological analysis, performed using the deformation test, did not reveal any reciprocal titres ≥320, indicating that strain TDA-040725-5T had minimal cross-reactivity to strains of recognized species of the genus Spiroplasma. Based on this evidence, strain TDA-040725-5T ( = CCTCC M 207170T = DSM 21848T) represents a novel species of the genus Spiroplasma, for which the name Spiroplasma eriocheiris sp. nov. is proposed, belonging to the novel Spiroplasma serological group XLIII.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence of strain TDA-040725-5T is DQ917753.
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Abbreviations: CCU colour-change unit DF deformation MP maximum-parsimony ML maximum-likelihood TD tremor disease TDA tremor disease agent TEM transmission electron microscopy
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