1887

Abstract

An oestradiol-degrading bacterium isolated at a sewage treatment plant in Tokyo was studied phenotypically, genotypically and phylogenetically. Analysis of its 16S rDNA sequence, DNA base composition, whole-cell fatty acid profile and isoprenoid quinone composition, as well as the presence of sphingoglycolipid, revealed that the isolate is a member of the genus . However, the sequence similarity of its 16S rDNA to those of known species was no higher than 97 %, implying that the isolate is distinctive. The results of DNA–DNA hybridization experiments and physiological characterization also indicated that the isolate represents a novel species, for which the name sp. nov. is proposed; strain ARI-1 (=JCM 11434 =ATCC BAA-531 =IFO 16725) is the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02301-0
2003-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/1/ijs530047.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02301-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Balkwill D. L., Drake G. R., Reeves R. H.7 other authors 1997; Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp. nov., Sphingomonas subterranea sp nov., and Sphingomonas stygia sp. nov. Int J Syst Bacteriol 47:191–201 [CrossRef]
    [Google Scholar]
  3. Baronti C., Curini R., D'Ascenzo G., Di Corcia A., Gentili A., Samperi R. 2000; Monitoring natural and synthetic estrogens at activated sludge sewage treatment plants and in a receiving river water. Environ Sci Technol 34:5059–5066 [CrossRef]
    [Google Scholar]
  4. Bligh E. J., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
    [Google Scholar]
  5. Desbrow C., Routledge E. J., Brighty G. C., Sumpter J. P., Waldock M. 1998; Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening. Environ Sci Technol 32:1549–1558 [CrossRef]
    [Google Scholar]
  6. Difco Laboratories 1998; Yeast media. In Difco Manual , 11th edn. pp 576–580 Detroit: Difco Laboratories;
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1995 phylip – Phylogeny Inference Package (version 3.57c). Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  9. Folmar L. C., Denslow N. D., Rao V., Chow M., Crain D. A., Enblom J., Marcino J., Guillette L. J. Jr 1996; Vitellogenin induction and reduced serum testosterone concentrations in feral male carp ( Cyprinus carpio ) captured near a major metropolitan sewage treatment plant. Environ Health Perspect 104:1096–1101 [CrossRef]
    [Google Scholar]
  10. Fujii K., Kikuchi S., Satomi M., Ushio-Sata N., Morita N. 2002; Degradation of 17β-estradiol by a gram-negative bacterium isolated from activated sludge in a sewage treatment plant in Tokyo, Japan. Appl Environ Microbiol 68:2057–2060 [CrossRef]
    [Google Scholar]
  11. Harries J. E., Sheahan D. A., Jobling S., Matthiessen P., Neall P., Routledge E. J., Rycroft R., Sumpter J. P., Tylor T. 1996; A survey of estrogenic activity in United Kingdom inland waters. Environ Toxicol Chem 15:1993–2002 [CrossRef]
    [Google Scholar]
  12. Harries J. E., Sheahan D. A., Jobling S., Matthiessen P., Neall P., Sumpter J. P., Tylor T., Zaman N. 1997; Estrogenic activity in five United Kingdom rivers detected by measurement of vitellogenesis in caged male trout. Environ Toxicol Chem 16:534–542 [CrossRef]
    [Google Scholar]
  13. Ishii Y., Okita S., Torigai M., Yun S. 2000; Determination of oestrogens in environmental water samples by LC/MS/MS. Bunseki Kagaku 49:753–758 in Japanese [CrossRef]
    [Google Scholar]
  14. Jobling S., Nolan M., Tyler C. R., Brighty G., Sumpter J. P. 1998; Widespread sexual disruption in wild fish. Environ Sci Technol 32:2498–2506 [CrossRef]
    [Google Scholar]
  15. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. B., Busse H.-J. 1997; Classification of “ Pseudomonas azotocolligans ” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583 [CrossRef]
    [Google Scholar]
  16. Kämpfer P., Witzenberger R., Denner E. B. M., Busse H.-J., Neef A. 2002; Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond. Syst Appl Microbiol 25:37–45 [CrossRef]
    [Google Scholar]
  17. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  18. Kumagai M., Fujimoto M., Kuninaka A. 1988; Determination of base composition of DNA by high performance liquid chromatography of its nuclease P1 hydrolysate. Nucleic Acids Symp Ser 19:65–68
    [Google Scholar]
  19. Matsui S., Takigami H., Matsuda T., Taniguchi N., Adachi J., Kawami H., Shimizu Y. 2000; Estrogen and estrogen mimics contamination in water and the role of sewage treatment. Water Sci Technol 42:12173–179
    [Google Scholar]
  20. Metcalfe C. D., Metcalfe T. L., Kiparissis Y., Koenig B. G., Khan C., Hughes R. J., Croley T. R., March R. E., Potter T. 2001; Estrogenic potency of chemicals detected in sewage treatment plant effluents as determined by in vivo assays with Japanese medaka ( Oryzias latipes ). Environ Toxicol Chem 20:297–308 [CrossRef]
    [Google Scholar]
  21. Nakada N. H., Takada H., Nyuunoya H., Nakamura M., Iguchi T. 2000; Identification of chemicals disrupting endocrine systems of aquatic biota in sewage effluent using GC-MS determination, chemical fractionation and recombinant yeast assay. In Proceedings of the 9th Symposium on Environmental Chemistry pp 122–123 Sapporo, Japan: in Japanese
    [Google Scholar]
  22. Nasu M., Goto M., Kato H., Oshima Y., Tanaka H. 2001; Study on endocrine disrupting chemicals in wastewater treatment plants. Water Sci Technol 43:2101–108
    [Google Scholar]
  23. Nicholson L. A., Morrow C. J., Corner L. A., Hodgson A. L. M. 1994; Phylogenic relationship of Fusobacterium necrophorum A, AB, and B biotypes based upon 16S rRNA gene sequence analysis. Int J Syst Bacteriol 44:315–319 [CrossRef]
    [Google Scholar]
  24. Nohynek L. J., Nurmiaho-Lassila E.-L., Suhonen E. L., Busse H.-J., Mohammadi M., Hantula J., Rainey F., Salkinoja-Salonen M. S. 1996; Description of chlorophenol-degrading Pseudomonas sp. strains KF1T, KF3, and NKF1 as a new species of the genus Sphingomonas Sphingomonas subarctica sp. nov. Int J Syst Bacteriol 46:1042–1055 [CrossRef]
    [Google Scholar]
  25. Routledge E. J., Sheahan D., Desbrow C., Brighty G. C., Waldock M., Sumpter J. P. 1998; Identification of estrogenic chemicals in STW effluent. 2. In vivo responses in trout and roach. Environ Sci Technol 32:1559–1565 [CrossRef]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Satomi M., Kimura B., Mizoi M., Sato T., Fujii T. 1997; Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47:832–836 [CrossRef]
    [Google Scholar]
  29. Shore L. S., Gurevitz M., Shemesh M. 1993; Estrogen as an environmental pollutant. Bull Environ Contam Toxicol 51:361–366
    [Google Scholar]
  30. Stolz A., Schmidt-Maag C., Denner E. B. M., Busse H.-J., Egli T., Kämpfer P. 2000; Description of Sphingomonas xenophaga sp. nov. for strains BN6T and N,N which degrade xenobiotic aromatic compounds. Int J Syst Evol Microbiol 50:35–41 [CrossRef]
    [Google Scholar]
  31. Tajima H., Tsujimura K., Yamaguchi M. 2000; Development of an analytical method of 17β-estradiol in river water by liquid chromatography/tandem mass spectrometry. Bunseki Kagaku 49:843–848 (in Japanese [CrossRef]
    [Google Scholar]
  32. Takeuchi M., Kawai F., Shimada Y., Yokota A. 1993; Taxonomic study of polyethylene glycol-utilizing bacteria: emended descriptions of the genus Sphingomonas and new description of Sphingomonas macrogoltabidus sp. nov., Sphingomonas sanguis sp. nov., and Sphingomonas terrae sp. nov. Syst Appl Microbiol 16:227–238 [CrossRef]
    [Google Scholar]
  33. Takeuchi M., Sawada H., Oyaizu H., Yokota A. 1994; Phylogenetic evidence for Sphingomonas and Rhizomonas as nonphotosynthetic members of the alpha-4 subclass of the Proteobacteria . Int J Syst Bacteriol 44:308–314 [CrossRef]
    [Google Scholar]
  34. Takeuchi M., Sakane T., Yanagi M., Yamasato K., Hamana K., Yokota A. 1995; Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp. nov. Int J Syst Bacteriol 45:334–341 [CrossRef]
    [Google Scholar]
  35. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera Sphingobium Novosphingobium and Sphingopyxis on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417
    [Google Scholar]
  36. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  38. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  39. Williams R. J., Jürgens M. D., Johnson A. C. 1999; Initial predictions of the concentrations and distribution of 17β-oestradiol, oestrone and ethinyl oestradiol in 3 English rivers. Water Res 33:1663–1671 [CrossRef]
    [Google Scholar]
  40. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov. Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119 [CrossRef]
    [Google Scholar]
  41. Yamada Y., Kuraishi I. 1982; Ubiquinone and menaquinone. In Biseibutsu no Kagakubunrui Jikkenhou pp 143–155Edited by Komagata K. Tokyo: Gakkai Shuppan Centre (in Japanese)
    [Google Scholar]
  42. Yokota A. 1999; Gram staining. In Biseibutsugaku-jikkenhou p 199Edited by Sugiyama J., Watanabe S., Oowada K., Kuroiwa T., Takahashi H., Tokuda G. Tokyo: Kodansha Scientific (in Japanese);
    [Google Scholar]
  43. Yrjälä K., Suomalainen S., Suhonen E. L., Kilpi S., Paulin L., Romantschuk M. 1998; Characterization and reclassification of an aromatic- and chloroaromatic-degrading Pseudomonas sp., strain HV3, as Sphingomonas sp. HV3. Int J Syst Bacteriol 48:1057–1062 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02301-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02301-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error