1887

Abstract

The taxonomic status of an actinobacterium that changed colour during growth, strain 42H12-1, isolated from deep-sea sediment collected from the Atlantic Ocean, was established using a combination of genotypic and phenotypic data. Strain 42H12-1 formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus . The highest sequence similarity by analysis was to CP153-2 (98.5 %) and the second-highest sequence similarity was to AA-826 (97.5 %). DNA–DNA relatedness of only 12 % ( 1.82 %) between strain 42H12-1 and . DSM 16678 differentiated them as members of separate genomic species. Colonies of strain 42H12-1 were black on oligotrophic medium, but orange to red, turning black, on copiotrophic medium. The peptidoglycan contained -diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and an unknown aminophospholipid. The predominant menaquinone was MK-9(H). The major fatty acids were iso-C and Cω8. The DNA GC content was 72.3±1 mol%. Strain 42H12-1 ( = DSM 45201  = CGMCC 4.5581) is assigned as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • China Ocean Mineral Resources Research and Development Association fund (Award DYXM-115-02-2-8)
  • National High Technology R&D Program of China (Award 2007AA091904)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023085-0
2011-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1710.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023085-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Cerny G. 1978; Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:113–122 [View Article]
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
    [Google Scholar]
  8. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  9. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp. 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  11. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication no. 6) pp. 227–291 Edited by Dietz A., Thayer J. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  14. Mevs U., Stackebrandt E., Schumann P., Gallikowski C. A., Hirsch P. 2000; Modestobacter multiseptatus gen. nov., sp. nov., a budding actinomycete from soils of the Asgard Range (Transantarctic Mountains). Int J Syst Evol Microbiol 50:337–346[PubMed] [CrossRef]
    [Google Scholar]
  15. Normand P. 2006; Geodermatophilaceae fam. nov., a formal description. Int J Syst Evol Microbiol 56:2277–2278 [View Article][PubMed]
    [Google Scholar]
  16. Reddy G. S., Potrafka R. M., Garcia-Pichel F. 2007; Modestobacter versicolor sp. nov., an actinobacterium from biological soil crusts that produces melanins under oligotrophy, with emended descriptions of the genus Modestobacter and Modestobacter multiseptatus Mevs et al. 2000. Int J Syst Evol Microbiol 57:2014–2020 [View Article][PubMed]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  18. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  21. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  22. Xu J., Wang Y., Xie S. J., Xu J., Xiao J., Ruan J. S. 2009; Streptomyces xiamenensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 59:472–476 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023085-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023085-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Supplementary material 4

PDF

Supplementary material 5

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error