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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 6

sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a -cultivated field Free

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated THG 15, was isolated from soil of a field cultivated with in Okcheon province, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain THG 15 grew optimally at 25–30 °C and at pH 7 in the absence of NaCl on nutrient agar. Strain THG 15 displayed β-glucosidase (aesculinase) activity that was responsible for its ability to transform ginsenoside Rb (one of the dominant active components of ginseng) into compound K via Rd and F. On the basis of 16S rRNA gene sequence similarities, strain THG 15 was shown to belong to the family and was most closely related to PSD1-4 (97.7 % sequence similarity), JS6-6 (97.5 %) and LMG 4025 (97.3 %). The G+C content of the genomic DNA was 35.7 mol%. The major menaquinone was MK-6 and the major fatty acids were iso-C (50.3 %), iso-C 3-OH (21.9 %), summed feature 4 (comprising Cω7 and/or iso-C 2-OH; 9.5 %) and iso-Cω9 (9.3 %). DNA sequence analysis and chemotaxonomic data supported the affiliation of strain THG 15 to the genus . DNA–DNA relatedness values between strain THG 15 and its closest phylogenetic neighbours were <15 %. Strain THG 15 could be differentiated genotypically and phenotypically from recognized species of the genus . The isolate therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is THG 15 ( = KACC 14527  = JCM 16719).

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Funding
This study was supported by the:
  • Rural Development Administration (Award PJ0070902009)
  • Kyung Hee University (Award 20100177)
© 2011 IUMS
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2011-06-01
2024-04-20
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Chryseobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a Rhus vernicifera-cultivated field
Int J Syst Evol Microbiol 61, 1430 (2011); https://doi.org/10.1099/ijs.0.023614-0
/content/journal/ijsem/10.1099/ijs.0.023614-0
/content/journal/ijsem/10.1099/ijs.0.023614-0
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