1887

Abstract

Two strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to belong to two separate taxa. Phylogenetic analysis of full-length 16S rRNA gene sequences showed that the strains were both related to, but distinct from, the type strain of . Two novel species, sp. nov. and sp. nov., are proposed to accommodate these strains. Both strains were saccharolytic and produced acetic and succinic acids, with lesser amounts of lactic and isovaleric acids, as end products of fermentation, and both were sensitive to 20 % bile. The principal cellular long-chain fatty acids of both strains were ai-C, 3-OH i-C, 3-OH C, i-C and C. The DNA G+C contents of the type strains of (W1435  = DSM 22504  = CCUG 57946) and (W2052  = DSM 22613 = CCUG 57945) were 43 and 41 mol%, respectively. The two species could be differentiated by gelatin hydrolysis, cellobiose and ribose fermentation, and production of β-glucosidase.

Funding
This study was supported by the:
  • Guy’s and St Thomas’ Charity (Award R050724)
  • Department of Health via the National Institute for Health Research (NIHR)
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2011-04-01
2024-03-28
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