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Volume 53, Issue 3

sp. nov., isolated from a salt lake on the coast of the East Sea in Korea Free

Abstract

A Gram-positive, rod-shaped, endospore-forming, halophilic bacterium (strain HSL-3) was isolated from a salt lake near Hwajinpo beach on the East Sea in Korea and was subjected to a polyphasic taxonomic study. Strain HSL-3 grew optimally in the presence of 2–10 % (w/v) NaCl. Strain HSL-3 showed poor growth in the absence of NaCl and grew in the presence of less than 23 % NaCl. The cell wall peptidoglycan type of strain HSL-3 was A4 based on -orn–-Asp. The predominant menaquinone found in strain HSL-3 was menaquinone-7 (MK-7). Strain HSL-3 had a cellular fatty acid profile containing large amounts of branched fatty acids; the major fatty acids were anteiso-C, iso-C and iso-C. The DNA G+C content of strain HSL-3 was 45 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain HSL-3 falls within the radiation of the cluster comprising species. Strain HSL-3 exhibited levels of 16S rDNA similarity of 97·4–98·4 % to the type strains of species. Levels of DNA–DNA relatedness between strain HSL-3 and the type strains of all validly named species were in the range 7·3–9·2 %. On the basis of phenotypic and phylogenetic data and the genomic distinctiveness, strain HSL-3 (=KCCM 41590=JCM 11546) should be placed in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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Keyword(s): MA, marine agar 2216 and MB, marine broth 2216
SGM
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2003-05-01
2024-04-19
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Halobacillussalinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea
Int J Syst Evol Microbiol 53, 687 (2003); https://doi.org/10.1099/ijs.0.02421-0
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