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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 9

gen. nov., sp. nov., an aerobic bacteriochlorophyll -containing alphaproteobacterium isolated from Antarctic sandy intertidal sediment Free

Abstract

A novel Gram-negative, non-motile bacterium, designated strain ZS2-28, was isolated from sandy intertidal sediment samples collected from the coastal regions of the Chinese Antarctic Zhongshan Station on the Larsemann Hills, Princess Elizabeth Land, East Antarctica. Strain ZS2-28 was obligately heterotrophic, strictly aerobic, psychrotolerant (growth occurred at 0–33 °C) and moderately halophilic (optimal growth in 7–8 % NaCl). A single major peak at 872–874 nm in the infrared absorption spectrum indicated the presence of bacteriochlorophyll . Poly-β-hydroxybutyrate accumulation and slime production were also detected. The predominant cellular fatty acid was Cω7, with C 3-OH, C, C cyclo, Cω8 cyclo and summed feature 3 (Cω7 and/or iso-C 2-OH) present in smaller amounts. The respiratory quinone was Q-10. The main polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminolipid. The G+C content of the genomic DNA was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZS2-28 formed a distinct evolutionary lineage within the clade containing members of the genera , and of the class . On the basis of its phylogenetic position, as well as its phenotypic and chemotaxonomic characteristics, strain ZS2-28 represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is ZS2-28 ( = CGMCC 1.8894  = LMG 24863).

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Funding
This study was supported by the:
  • National High Technology R&D Program of China (Award 2007AA09Z434)
  • National Key Technology R&D Program of China (Award 2006BAB18B07)
  • National Natural Science Foundation of China (Award 30500001 and 30500377)
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award 2005DKA21209)
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2011-09-01
2024-04-18
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Roseicitreum antarcticum gen. nov., sp. nov., an aerobic bacteriochlorophyll a-containing alphaproteobacterium isolated from Antarctic sandy intertidal sediment
Int J Syst Evol Microbiol 61, 2173 (2011); https://doi.org/10.1099/ijs.0.024885-0
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