@article{mbs:/content/journal/ijsem/10.1099/ijs.0.025064-0, author = "Shimane, Yasuhiro and Hatada, Yuji and Minegishi, Hiroaki and Echigo, Akinobu and Nagaoka, Syuhei and Miyazaki, Masayuki and Ohta, Yukari and Maruyama, Tadashi and Usami, Ron and Grant, William D. and Horikoshi, Koki", title = "Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "9", pages = "2266-2270", doi = "https://doi.org/10.1099/ijs.0.025064-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.025064-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Strain YSM-79T was isolated from commercial salt made from seawater in Yonaguni island, Okinawa, Japan. The strain is an aerobic, Gram-negative, chemo-organotrophic and extremely halophilic archaeon. Cells are short rods that lyse in distilled water. Growth occurs at 1.5–5.3 M NaCl (optimum 2.5–3.0 M), pH 5.0–8.8 (optimum pH 5.2–6.3) and 20–55 °C (optimum 40 °C). Mg2+ is required for growth, with maximum growth at 200–300 mM Mg2+. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, sulfated diglycosyl diether-1 and five unidentified glycolipids. The G+C content of the DNA was 64 mol%. On the basis of 16S rRNA gene sequence analysis, strain YSM-79T was determined to be a member of the family Halobacteriaceae, with the closest related genus being Halobacterium (94 % sequence identity). In addition, the rpoB′ gene sequence of strain YSM-79T had <88 % sequence similarity to those of other members of the family Halobacteriaceae. The results of phenotypic, chemotaxonomic and phylogenetic analysis suggested that strain YSM-79T should be placed in a new genus, Salarchaeum gen. nov., as a representative of Salarchaeum japonicum sp. nov. The type strain is YSM-79T ( = JCM 16327T  = CECT 7563T).", }