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f gen. nov., sp. nov., a moderately halophilic, Gram-positive, facultative anaerobe isolated from subsurface saline soil

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  • Authors: Xiaowei Wang1,2 ,  Yanfen Xue1 , Yanhe Ma1
    • VIEW AFFILIATIONS
    • 1 1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 2 2Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China
  • Yanfen Xue [email protected]
  • First Published Online: 01 May 2011, International Journal of Systematic and Evolutionary Microbiology 61: 1127-1132, doi: 10.1099/ijs.0.025338-0
  • Subject: New Taxa - Firmicutes And Related Organisms
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gen. nov., sp. nov., a moderately halophilic, Gram-positive, facultative anaerobe isolated from subsurface saline soil, Page 1 of 1

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  • A Gram-stain-positive, rod-shaped, non-sporulating, motile and moderately halophilic bacterium, designated strain H96B60, was isolated from a saline soil sample of the Qaidam basin, China. The strain was facultatively anaerobic. Major end products formed from glucose fermentation were acetate, ethanol and lactic acid. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone component was menaquinone-6 (MK-6). The predominant cellular fatty acids were C, anteiso-C and anteiso-C. The genomic DNA G+C content of strain H96B60 was 36.2 mol%. Phylogenetic analysis based on comparative 16S rRNA gene sequences indicated that strain H96B60 represented a novel phyletic lineage within the family and was related most closely to species (96.1–96.4 % similarity). Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain H96B60 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is H96B60 ( = DSM 22440  = CGMCC 1.7733).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain H96B60 is FJ746578.

  • A supplementary figure and two supplementary tables are available with the online version of this paper.

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