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Volume 53, Issue 4

gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent Free

Abstract

A moderately thermophilic, anaerobic, endospore-forming bacterium (strain SC 562) was isolated from a hydrothermal vent chimney located at 13° N on the East-Pacific Rise at a depth of 2650 m. Cells of strain SC 562 were straight to slightly curved rods, which were 0·7–0·9 μm in diameter and 3·5–6·0 μm in length with peritrichous flagella. Strain SC 562 formed round, refractile endospores in terminally swollen sporangia. The temperature range for growth was 33–60 °C, with an optimum at 50 °C. The pH range for growth was 4·8–8·5, with an optimum at pH 6·5–6·8. Growth of strain SC 562 was observed at NaCl concentrations ranging from 1·5 to 6 % (w/v). The substrates utilized by strain SC 562 included casein, peptone, albumin, yeast extract, beef extract, alanine plus proline and starch. Glucose, maltose, pyruvate, valine and arginine each slightly stimulated growth in the presence of yeast extract. The products of glucose fermentation were ethanol, acetate, H and CO. Strain SC 562 reduced elemental sulfur to hydrogen sulfide. The G+C content of the DNA of strain SC 562 was 24 mol%. 16S rDNA sequence analysis revealed that the isolated organism belonged to cluster XI of the subphylum. On the basis of its physiological properties and phylogenetic analyses, it is proposed that strain SC 562 represents the sole species of a novel genus, ; the name is proposed for strain SC 562 (=DSM 15285 =UNIQEM 215).

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2003-07-01
2024-04-20
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References

  1. Alain K., Marteinsson V. T., Miroshnichenko M. L., Bonch-Osmolovskaya E. A., Prieur D., Birrien J. L. 2002a; Marinitoga piezophila sp. nov., a rod-shaped, thermo-piezophilic bacterium isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52:1331–1339 [CrossRef]
     [Google Scholar]
  2. Alain K., Pignet P., Zbinden M.8 other authors 2002b; Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52:1621–1628 [CrossRef]
     [Google Scholar]
  3. Antoine E., Cilia V., Meunier J. R., Guezennec J., Lesongeur F., Barbier G. 1997; Thermosipho melanesiensis sp. nov., a new thermophilic anaerobic bacterium belonging to the order Thermotogales , isolated from deep-sea hydrothermal vents in the southwestern Pacific Ocean. Int J Syst Bacteriol 47:1118–1123 [CrossRef]
     [Google Scholar]
  4. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
     [Google Scholar]
  5. Jeanthon C. 2000; Molecular ecology of hydrothermal vent microbial communities. Antonie van Leeuwenhoek 77:117–133 [CrossRef]
     [Google Scholar]
  6. Li Y., Mandelco L., Wiegel J. 1993; Isolation and characterization of a moderately thermophilic anaerobic alkaliphile, Clostridium paradoxum sp. nov. Int J Syst Bacteriol 43:450–460 [CrossRef]
     [Google Scholar]
  7. Li Y., Engle M., Weiss N., Mandelco L., Wiegel J. 1994; Clostridium thermoalcaliphilum sp. nov., an anaerobic and thermotolerant facultative alkaliphile. Int J Syst Bacteriol 44:111–118 [CrossRef]
     [Google Scholar]
  8. Slobodkin A., Reysenbach A.-L., Strutz N., Dreier M., Wiegel J. 1997; Thermoterrabacterium ferrireducens gen. nov., sp. nov. a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring. Int J Syst Bacteriol 47:541–547 [CrossRef]
     [Google Scholar]
  9. Slobodkin A. I., Tourova T. P., Kuznetsov B. B., Kostrikina N. A., Chernyh N. A., Bonch-Osmolovskaya E. A. 1999; Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium. Int J Syst Bacteriol 49:1471–1478 [CrossRef]
     [Google Scholar]
  10. Slobodkin A., Campbell B., Cary S. C., Bonch-Osmolovskaya E., Jeanthon C. 2001; Evidence for the presence of thermophilic Fe(III)-reducing microorganisms in deep-sea hydrothermal vents at 13° N (East Pacific Rise. FEMS Microbiol Ecol 36:235–243
     [Google Scholar]
  11. Sokolova T. G., Gonzales J. M., Kostrikina N. A., Chernyh N. A., Tourova T. P., Kato C., Bonch-Osmolovskaya E. A., Robb F. T. 2001; Carboxydobrachium pacificum gen. nov., sp. nov., a new anaerobic, thermophilic, CO-utilizing marine bacterium from Okinawa Trough. Int J Syst Evol Microbiol 51:141–149
     [Google Scholar]
  12. Takai K., Horikoshi K. 2000; Thermosipho japonicus sp. nov., an extremely thermophilic bacterium isolated from a deep-sea hydrothermal vent in Japan. Extremophiles 4:9–17 [CrossRef]
     [Google Scholar]
  13. Wery N., Lesongeur F., Pignet P., Derennes V., Cambon-Bonavita M. A., Godfroy A., Barbier G. 2001a; Marinitoga camini gen. nov., sp. nov. a rod-shaped bacterium belonging to the order Thermotogales , isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:495–504
     [Google Scholar]
  14. Wery N., Moricet J.-M., Cueff V., Jean J., Pignet P., Lesongeur F., Cambon-Bonavita M.-A., Barbier G. 2001b; Caloranaerobacter azorensis gen. nov., sp. nov. an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:1789–1796 [CrossRef]
     [Google Scholar]
  15. Woese C. R., Achenbach L., Rouvière P., Mandelco L. 1991; Archaeal phylogeny: reexamination of the phylogenetic position of Archaeoglobus fulgidus in light of certain composition-induced artifacts. Syst Appl Microbiol 14:364–371 [CrossRef]
     [Google Scholar]
  16. Wolin E. A., Wolin M. J., Wolfe R. S. 1963; Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886
     [Google Scholar]
  17. Zavarzina D. G., Tourova T. P., Kuznetsov B. B., Bonch-Osmolovskaya E. A., Slobodkin A. I. 2002; Thermovenabulum ferriorganovorum gen. nov. sp. nov., a novel thermophilic, anaerobic, endospore-forming bacterium. Int J Syst Evol Microbiol 52:1737–1743 [CrossRef]
     [Google Scholar]
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Tepidibacterthalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent
Int J Syst Evol Microbiol 53, 1131 (2003); https://doi.org/10.1099/ijs.0.02600-0
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