Halomonas stenophila sp. nov., a halophilic bacterium that produces sulphate exopolysaccharides with biological activity
Llamas, Inmaculada and Béjar, Victoria and Martínez-Checa, Fernando and Martínez-Cánovas, María José and Molina, Ignacio and Quesada, Emilia,, 61, 2508-2514 (2011),
doi = https://doi.org/10.1099/ijs.0.026369-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= We have undertaken a polyphasic taxonomic study of two halophilic, Gram-negative bacterial strains, N12T and B-100, that produce sulphated exopolysaccharides with biological activity. They were isolated from two different saline soil samples. Both strains grow at NaCl concentrations within the range 3–15 % (w/v) [optimum 5–10 % (w/v)], at 15–37 °C (optimum 20–32 °C) and at pH 6–8 (optimum pH 7–8). Their 16S rRNA gene sequences indicate that they belong to the genus Halomonas in the class Gammaproteobacteria. Their closest relative is Halomonas nitroreducens, to which our strains show maximum 16S rRNA gene sequence similarity values of 98.7 % (N12T) and 98.3 % (B-100). Their DNA G+C contents are 61.9 and 63.8 mol%, respectively. The results of DNA–DNA hybridizations showed 43.9 % relatedness between strain N12T and H. nitroreducens CECT 7281T, 30.5 % between N12T and Halomonas ventosae CECT 5797T, 39.2 % between N12T and Halomonas fontilapidosi CECT 7341T, 46.3 % between N12T and Halomonas maura CECT 5298T, 52.9 % between N12T and Halomonas saccharevitans LMG 23976T, 51.3 % between N12T and Halomonas koreensis JCM 12237T and 100 % between strains N12T and B-100. The major fatty acids of strain N12T are C12 : 0 3-OH (5.42 %), C15 : 0 iso 2-OH/C16 : 1ω7c (17.37 %), C16 : 0 (21.62 %) and C18 : 1ω7c (49.19 %). The proposed name for the novel species is Halomonas stenophila sp. nov. Strain N12T ( = CECT 7744T  = LMG 25812T) is the type strain.,
language=,
type=