1887

Abstract

A Gram-staining-positive, motile, rod-shaped, spore-forming bacterium, designated P9, was isolated from soil in Portugal. This organism was aerobic and catalase- and oxidase-positive. It had an optimum growth temperature of about 35 °C and an optimum growth pH of about 8.0–8.5, and grew in medium with 0–9 % (w/v) NaCl. The cell-wall peptidoglycan was of the A1α type, with -lysine as the diagnostic diamino acid. The major respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C (45.4 %), iso-C (22.0 %) and anteiso-C (11.2 %). The genomic DNA G+C content was about 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P9 was most closely related to DSM 18983 (96.8 %) and DSM 1297 (96.5 %). These two recognized species formed a coherent cluster with strain P9 that was supported by a bootstrap value of 99 %. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, strain P9 ( = DSM 23228 = LMG 25523) represents a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (FCT)/Fundo Europeu de Desenvolvimento Regional (FEDER) (Award POCI/BIA-BDE/60704/2004)
  • FCT (Award SFRH/BD/46212/2008)
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2012-03-01
2024-04-19
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References

  1. da Costa M. S., Nobre M. F., Wait R. 2006; Analysis of lipids from extremophilic bacteria. Methods Microbiol 35:127–159 [View Article]
    [Google Scholar]
  2. Felsenstein J. 1983; Parsimony in systematics: biological and statistical issues. Annu Rev Ecol Syst 14:313–333 [View Article]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  4. Hudson J. A., Morgan H. W., Daniel R. M. 1986; A numerical classification of some Thermus isolates. J Gen Microbiol 132:531–540
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  6. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. other authors 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [View Article][PubMed]
    [Google Scholar]
  7. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  9. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  11. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [View Article]
    [Google Scholar]
  12. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp. 411–414 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Steele T. W., Lanser J., Sangster N. 1990; Isolation of Legionella longbeachae serogroup 1 from potting mixes. Appl Environ Microbiol 56:49–53[PubMed]
    [Google Scholar]
  14. Tiago I., Chung A. P., Veríssimo A. 2004; Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. Appl Environ Microbiol 70:7378–7387 [View Article][PubMed]
    [Google Scholar]
  15. Yoon J.-H., Weiss N., Lee K.-C., Lee I.-S., Kang K. H., Park Y.-H. 2001; Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:2087–2093 [View Article][PubMed]
    [Google Scholar]
  16. Yoon J.-H., Kang S.-J., Schumann P., Oh T.-K. 2010; Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively. Int J Syst Evol Microbiol 60:15–20 [View Article][PubMed]
    [Google Scholar]
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