1887

Abstract

Two strains, designated B1-1 and B6-8, were isolated from the Geumho River and the Dalseo Stream in Korea. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class , their closest relatives being KCTC 12095, KCTC 12575, KACC 12605 and KACC 12910 with 16S rRNA gene sequence similarities of 95.3 –97.7 % to the two novel strains. Strains B1-1 and B6-8 shared a 16S rRNA gene sequence similarity value of 96.1 %. Cells of the two strains were Gram-reaction-negative, aerobic, non-motile, short rods or cocci. The predominant ubiquinone was Q-10. The major fatty acids were C Cω7, C and Cω8 cyclo for strain B1-1 and C Cω7, C, C 2-OH, and Cω8 cyclo for strain B6-8. The G+C contents of the genomic DNA of the strains B1-1 and B6-8 were 61.6 and 66.5 mol%, respectively. Based on the results of this polyphasic study, strains B1-1 ( = KCTC 12849  = DSM 18799) and B6-8 ( = KCTC 12850  = DSM 18800) represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively.

Funding
This study was supported by the:
  • 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology (Award MG08-0101-2-0)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.028894-0
2011-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/11/2577.html?itemId=/content/journal/ijsem/10.1099/ijs.0.028894-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  2. Bates R. G., Bower V. E. 1956; Alkaline solutions for pH control. Anal Chem 28:1322–1324 [View Article]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid- deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146 [View Article]
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Im W.-T., Yokota A., Kim M.-K., Lee S.-T. 2004; Kaistia adipata gen. nov., sp. nov., a novel α-proteobacterium. J Gen Appl Microbiol 50:249–254 [View Article][PubMed]
    [Google Scholar]
  10. Kim S.-J., Weon H.-Y., Kim Y.-S., Anandham R., Yoo S.-H., Park I.-C., Kwon S.-W. 2010; Kaistia terrae sp. nov., isolated from a wetland in Korea. Int J Syst Evol Microbiol 60:949–952 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  12. Kumar S., Tamura K., Nei M. 2004; mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  13. Lee H.-W., Yu H.-S., Liu Q.-M., Jung H.-M., An D.-S., Im W.-T., Jin F.-X., Lee S.-T. 2007; Kaistia granuli sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 57:2280–2283 [View Article][PubMed]
    [Google Scholar]
  14. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  16. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc;
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  18. Tarrand J. J., Gröschel D. H. M. 1982; Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 16:772–774[PubMed]
    [Google Scholar]
  19. Ten L. N., Im W.-T., Kim M.-K., Kang M. S., Lee S.-T. 2004; Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56:375–382 [View Article][PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  22. Weon H.-Y., Lee C.-M., Hong S.-B., Kim B.-Y., Yoo S.-H., Kwon S.-W., Go S.-J. 2008; Kaistia soli sp. nov., isolated from a wetland in Korea. Int J Syst Evol Microbiol 58:1522–1524 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.028894-0
Loading
/content/journal/ijsem/10.1099/ijs.0.028894-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error