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Volume 62, Issue Pt_3

sp. nov., a thermophilic, anaerobic bacterium capable of growth via electron shuttling between humic acid and Fe(III) Free

Abstract

An anaerobic, thermophilic, spore-forming bacterium (strain 64-FGQ) was isolated from a terrestrial hydrothermal spring from the Kamchatka peninsula, Russia. This strain utilized lactate as an electron donor, insoluble poorly crystalline Fe(III) oxide incorporated into alginate beads as a potential electron acceptor and 9,10-anthraquinone-2,6-disulfonate (AQDS) as an electron-shuttling compound. Vegetative cells of strain 64-FGQ were Gram-stain-positive, peritrichously flagellated, motile, straight rods, 0.3–0.5 µm in diameter and 2.0–5.0 µm long, growing singly or forming short chains. Cells formed round refractive endospores in terminal swollen sporangia. The temperature range for growth was 46–70 °C, with an optimum at 65 °C. The pH range for growth was 5.5–8.5, with an optimum at pH 7.0. The substrates utilized by strain 64-FGQ in the presence of AQDS as an electron acceptor included lactate, malate, succinate, glycerol and yeast extract. The strain fermented galactose, fructose, maltose, sucrose, pyruvate and peptone. Strain 64-FGQ used AQDS, humic acid, thiosulfate, nitrate and perchlorate as electron acceptors for growth. Fe(III) was not directly reduced, but strain 64-FGQ was able to grow and reduce Fe(III) oxide in the presence of small amounts of AQDS or humic acid as electron-shuttling compounds. The G+C content of the DNA of strain 64-FGQ was 51 mol%. 16S rRNA gene sequence analysis placed the isolate in the genus , with the type strain of as its closest relative (97.2 % similarity). Based on phylogenetic analysis and physiological characteristics, strain 64-FGQ is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is 64-FGQ ( = DSM 23265 = VKM B-2603).

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Funding
This study was supported by the:
  • Russian Foundation for Basic Research (Award 09-04-00251-a)
  • Russian Academy of Sciences
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2012-03-01
2024-04-19
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Moorella humiferrea sp. nov., a thermophilic, anaerobic bacterium capable of growth via electron shuttling between humic acid and Fe(III)
Int J Syst Evol Microbiol 62, 613 (2012); https://doi.org/10.1099/ijs.0.029009-0
/content/journal/ijsem/10.1099/ijs.0.029009-0
/content/journal/ijsem/10.1099/ijs.0.029009-0
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