1887

Abstract

Gram-negative bacteria were isolated from knots induced by in olive trees ( L.). A total of nine endophytic bacterial strains were isolated, each from inside a different tree knot. Biochemical characterization indicated that all the strains belong to the family . Phylogenetic analyses of the 16S rRNA genes of these novel isolates revealed that they formed a homogeneous cluster within species. DNA signatures of these isolates were identical to those described for the genus . The strains formed a homogeneous group as shown by DNA–DNA hybridization analysis and numerical analysis of phenotypic data, clearly differentiated from all species of with validly published names. The data provide strong evidence of the differentiation of these strains from the most closely related species. Therefore, these isolates represent a novel species, for which the name sp. nov. is proposed. The isolates are available at CFBP, CECT and ATCC. The G+C content is 52±0·5 mol%. The type strain is CFBP 6631 (=A37=ATCC 700880=CECT 5263).

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2004-11-01
2024-03-29
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Billing E., Baker L. A. E. 1963; Characteristics of Erwinia -like organisms found in plant material. J Appl Bacteriol 26:58–65 [CrossRef]
    [Google Scholar]
  3. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli . J Mol Biol 148:107–127 [CrossRef]
    [Google Scholar]
  4. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol 115:904–911
    [Google Scholar]
  5. Descamps P., Véron M. 1981; Une méthode de choix des caractères d'identification basée sur le théorème de Bayes et la mesure de l'information. Ann Microbiol (Paris) 132B:157–170 (in French
    [Google Scholar]
  6. Dye D. W. 1969; A taxonomic study of the genus Erwinia . III. The “ herbicola ” group. N Z J Sci 12:223–236
    [Google Scholar]
  7. Ewing W. H., Fife M. A. 1971 Enterobacter agglomerans, the Herbicola-Lathyri Bacteria Atlanta, GA: Centers for Disease Control;
    [Google Scholar]
  8. Ewing W. H., Fife M. A. 1972; Enterobacter agglomerans (Beijerinck) comb. nov. (the herbicola-lathyri bacteria). Int J Syst Bacteriol 22:4–11 [CrossRef]
    [Google Scholar]
  9. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13 [CrossRef]
    [Google Scholar]
  10. García de los Ríos J. E. 1999; Retama sphaerocarpa (L.) Boiss. a new host of Pseudomonas savastanoi . Phytopathol Mediterr 38:54–60
    [Google Scholar]
  11. Gavini F., Mergaert J., Beji A., Mielcarek C., Izard D., Kersters K., De Ley J. 1989; Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen.nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int J Syst Bacteriol 39:337–345 [CrossRef]
    [Google Scholar]
  12. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr Microbiol 4:325–330 [CrossRef]
    [Google Scholar]
  13. Hauben L., Moore E. R. B., Vauterin L., Steenackers M., Mergaert J., Verdonck L., Swings J. 1998; Phylogenetic position of phytopathogens within the Enterobacteriaceae . Syst Appl Microbiol 21:384–397 [CrossRef]
    [Google Scholar]
  14. Huelsenbeck J. P., Ronquist F. 2001; mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 [CrossRef]
    [Google Scholar]
  15. Lelliott R. A., Dickey R. S. 1984; Genus VII. Erwinia Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith, 1920, 209AL . In Bergey's Manual of Systematic Bacteriology vol  1 pp  469–476 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  17. Mergaert J., Verdonck L., Kersters K. 1993; Transfer of Erwinia ananas (synonym, Erwinia uredovora ) and Erwinia stewartii to the genus Pantoea emend. as Pantoea ananas (Serrano 1928) comb.nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoeastewartii subsp. indologenes subsp. nov. Int J Syst Bacteriol 43:162–173 [CrossRef]
    [Google Scholar]
  18. Mergaert J., Hauben L., Cnockaert M. C., Swings J. 1999; Reclassification of non-pigmented Erwinia herbicola strains from trees as Erwinia billingiae sp. nov. Int J Syst Bacteriol 49:377–383 [CrossRef]
    [Google Scholar]
  19. Murray M. G., Thompson W. F. 1989; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology vol 1 pp. 2.4.1–2.4.5 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  20. Notredame C., Higgins D. G., Heringa J. 2000; T-Coffee: a novel method for fast and accurate multiple sequence alignment. J Mol Biol 302:205–217 [CrossRef]
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy: the Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  23. Sutra L., Christen R., Bollet C., Simoneau P., Gardan L. 2001; Samsonia erythrinae gen. nov. sp. nov. isolated from bark necrotic lesions of Erythrina sp., and discrimination of plant-pathogenic Enterobacteriaceae by phenotypic features. Int J Syst Evol Microbiol 511291–1304
    [Google Scholar]
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