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Volume 54, Issue 6

sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations Free

Abstract

The taxonomic position of a halo- and organo-sensitive, oligotrophic soil bacterium, strain S213, was investigated. Cells were Gram-negative, non-motile, strictly aerobic, yellow-pigmented rods of short to medium length on diluted nutrient broth. When 0·1–0·4 % (w/v) NaCl was added to diluted media composed of peptone and meat extract, growth was inhibited with increasing NaCl concentration and the cells became long aberrant forms. When 6 mM CaCl was added, the cells grew quite normally and aberrant cells were no longer found at 0·1–0·5 % (w/v) NaCl. Chemotaxonomically, strain S213 contains chemical markers that indicate its assignment to the : the presence of ubiquinone Q-10 as the predominant respiratory quinone, C and C as major fatty acids, C 2-OH as the major 2-hydroxy fatty acid and sphingoglycolipids. 16S rRNA gene sequence analysis indicated that strain S213 belongs to the genus , exhibiting high sequence similarity to the 16S rRNA gene sequences of IFO 15500 (98·3 %), IFO 15498 (98·0 %), IFO 15499 (97·9 %) and DSM 1805 (97·8 %). The results of DNA–DNA hybridization experiments and its phenotypic characteristics clearly distinguished the strain from its nearest neighbours and demonstrate that strain S213 represents a novel species, for which the name sp. nov. is proposed. The type strain is S213 (=JCM 12082=CIP 107926).

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2004-11-01
2024-04-24
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Sphingomonas oligophenolica sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations
Int J Syst Evol Microbiol 54, 2185 (2004); https://doi.org/10.1099/ijs.0.02959-0
/content/journal/ijsem/10.1099/ijs.0.02959-0
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