1887

Abstract

Two strains of curved bacteria, 7-1 and 7-2, isolated from well water, were phylogenetically examined to determine their taxonomic position. Strain 7-1 is a Gram-negative, slightly curved rod. Analysis of the 16S rRNA gene sequence showed that strain 7-1 formed a cluster with [] and [] . It has some similar characteristics to [] and [] , but has sufficient distance to separate it from other genera. DNA–DNA hybridization analysis, as well as chemotaxonomic and morphological studies, demonstrated that strain 7-1, [] and [] belong to a new genus, gen. nov. Strain 7-1 (=IAM 15033=ATCC BAA-807) is classified as the type strain of gen. nov., sp. nov., and [] and [] are classified as comb. nov. and comb. nov., respectively. Strain 7-2 is a Gram-negative spirillum. Phylogenetic study based on the 16S rRNA gene sequences showed that it formed a cluster with the members of the genus , [] and [] . The classification is therefore proposed of strain 7-2 (=IAM 15032=ATCC BAA-806) as the type strain of sp. nov., and [] and [] are transferred to the genus as comb. nov. and comb. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02975-0
2004-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542223.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02975-0&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  2. Aragno M., Schlegel H. G. 1978; Aquaspirillum autotrophicum , a new species of hydrogen-oxidizing, facultatively autotrophic bacteria. Int J Syst Bacteriol 28:112–116 [CrossRef]
    [Google Scholar]
  3. Baldani J. I., Baldani V. L. D., Seldin L., Döbereiner J. 1986; Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. Int J Syst Bacteriol 36:86–93 [CrossRef]
    [Google Scholar]
  4. Baldani J. I., Pot B., Kirchhof G. 8 other authors 1996; Emended description of Herbaspirillum ; inclusion of [ Pseudomonas ] rubrisubalbicans , a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. Int J Syst Bacteriol 46:802–810 [CrossRef]
    [Google Scholar]
  5. Ding L., Yokota A. 2002; Phylogenetic analysis of the genus Aquaspirillum based on 16S rRNA gene sequences. FEMS Microbiol Lett 212:165–169 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Hamana K., Sakane T., Yokota A. 1994; Polyamine analysis of the genera Aquaspirillum , Magnetospirillum , Oceanospirillum and Spirillum . J Gen Appl Microbiol 40:75–82 [CrossRef]
    [Google Scholar]
  8. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15:210–213 [CrossRef]
    [Google Scholar]
  9. Hiraishi A., Hoshino Y., Satoh T. 1991; Rhodoferax fermentens gen. nov. sp. nov. a phototrophic purple nonsulfur bacterium previously referred to as the “ Rhodocyclus gelatinosus -like” group. Arch Microbiol 155330–336
    [Google Scholar]
  10. Hylemon P. B., Wells J. S. Jr, Krieg N. R., Jannasch H. W. 1973; The genus Spirillum : a taxonomic study. Int J Syst Bacteriol 23:340–380 [CrossRef]
    [Google Scholar]
  11. IAM 1998 Catalogue of Strains pp  277–285 Tokyo: The Microbiology Research Foundation;
    [Google Scholar]
  12. Irgens R. L., Gosink J. J., Staley J. T. 1996; Polaromonas vacuolata gen. nov., sp. nov. a psychrophilic, marine, gas vacuolate bacterium from Antarctica. Int J Syst Bacteriol 46:822–826 [CrossRef]
    [Google Scholar]
  13. Kersters K., Ludwig W., Vancanneyt M., De Vos P., Gillis M., Schleifer K.-H. 1996; Recent change in the classification of the pseudomonads: an overview. Syst Appl Microbiol 19:465–477 [CrossRef]
    [Google Scholar]
  14. Kirchhof G., Eckert B., Stoffels M., Baldani J. I., Reis V. M., Hartmann A. 2001; Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. Int J Syst Evol Microbiol 51:157–168
    [Google Scholar]
  15. Krieg N. R. 1984; Genus Aquaspirillum Hylemon, Wells, Krieg and Jannasch 1973, 361AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  72–90 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Leifson E. 1962; The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium , Flavobacterium , Spirillum and Pseudomonas . Int Bull Bacteriol Nomencl Taxon 12:161–170
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Meyer S. A., Schleifer K. H. 1978; Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Arch Microbiol 117:183–188 [CrossRef]
    [Google Scholar]
  19. Pot B., Willems A., Gillis M., De Ley J. 1992; Intra- and intergeneric relationships of the genus Aquaspirillum : Prolinoborus , a new genus for Aquaspirillum fasciculus , with the species Prolinoborus fasciculus comb. nov. Int J Syst Bacteriol 42:44–57 [CrossRef]
    [Google Scholar]
  20. Reinhold-Hurek B., Hurek T. 1998; Life in grasses: diazotrophic endophytes. Trends Microbiol 6:139–144 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sakane T., Yokota A. 1994; Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum , Magnetospirillum and Oceanospirillum . Syst Appl Microbiol 17:128–134 [CrossRef]
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  25. Stoltzfus J. R., So R., Malarvithi P. P., Ladha J. K., de Bruijin F. J. 1997; Isolation of endophytic bacteria from rice and assessment of their potential for supplying rice with biologically fixed nitrogen. Plant Soil 194:25–36 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  27. Uchino Y., Yokota A., Sugiyama J. 1997; Phylogenetic position of the marine subdivision of Agrobacterium species based on 16S rRNA sequence analysis. J Gen Appl Microbiol 43:243–247 [CrossRef]
    [Google Scholar]
  28. Valverde A., Velazquez E., Gutierrez C., Cervantes E., Ventosa A., Igual J. M. 2003; Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris . Int J Syst Evol Microbiol 53:1979–1983 [CrossRef]
    [Google Scholar]
  29. Wen A., Fegan M., Hayward C., Chakraborty S., Sly L. I. 1999; Phylogenetic relationships among members of the Comamonadaceae , and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al 1987) gen. nov., comb. nov.. Int J Syst Bacteriol 49:567–576 [CrossRef]
    [Google Scholar]
  30. Zehr J. P., McReynolds L. A. 1989; Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii . Appl Environ Microbiol 55:2522–2526
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02975-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02975-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error