1887

Abstract

Two novel Gram-stain-negative, aerobic strains (ZXM100 and ZXM098) were isolated from seawater in the coastal region of Qingdao (36.027° N 120.184° E), China, during a massive green algae bloom. Cells were ovoid to irregular short rods and lacked flagella. Poly-β-hydroxybutyrate was accumulated. Cells did not contain bacteriochlorophyll . The isolates grew at NaCl concentrations of 3–9 % and 22–28 °C. Cω7, C and C were the major fatty acid components. The polar lipids of ZXM100 were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, a single lipid and an unidentified aminolipid. The predominant isoprenoid quinone of ZXM100 was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains belonged to the family . Based on the results obtained in this study, strain ZXM100 was considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is ZXM100 ( = LMG 24861 = CGMCC 1.10234); the DNA GC content of the type strain is 54.6 mol%.

Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 863 Program, no. 2007AA09Z434)
  • National Natural Science Foundation of China (Award no. 40876067)
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2012-05-01
2024-03-28
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References

  1. Beveridge T. J., Lawrence J. R., Murray R. G. E. 2007; Sampling and staining for light microscopy. In Methods for General and Molecular Microbiology pp. 19–33 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Bosshard P. P., Santini Y., Grüter D., Stettler R., Bachofen R. 2000; Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis. FEMS Microbiol Ecol 31:173–182 [View Article][PubMed]
    [Google Scholar]
  3. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology pp. 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Buchan A., González J. M., Moran M. A. 2005; Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71:5665–5677 [View Article][PubMed]
    [Google Scholar]
  5. Choi D. H., Cho B. C. 2006; Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. Int J Syst Evol Microbiol 56:1869–1873 [View Article][PubMed]
    [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  7. Gao J., Pan H., Xiao T., Barbier G., Wang Z., Yue H., Sun S., Nitsche S., Bernadac A. other authors 2006; Isolation and characterization of novel marine Roseobacter clade members producing unique intracellular chromium-rich aggregates. Res Microbiol 157:714–719 [View Article][PubMed]
    [Google Scholar]
  8. Ivanova E. P., Gorshkova N. M., Sawabe T., Zhukova N. V., Hayashi K., Kurilenko V. V., Alexeeva Y., Buljan V., Nicolau D. V. other authors 2004; Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina). Int J Syst Evol Microbiol 54:475–480 [View Article][PubMed]
    [Google Scholar]
  9. Kim B.-Y., Weon H.-Y., Son J.-A., Lee C.-M., Hong S.-B., Jeon Y.-A., Koo B.-S., Kwon S.-W. 2009; Thalassobacter arenae sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 59:487–490 [View Article][PubMed]
    [Google Scholar]
  10. Kurahashi M., Yokota A. 2007; Tateyamaria omphalii gen. nov., sp. nov., an α-Proteobacterium isolated from a top shell Omphalius pfeifferi pfeifferi . Syst Appl Microbiol 30:371–375 [View Article][PubMed]
    [Google Scholar]
  11. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P. 2000; Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. Int J Syst Evol Microbiol 50:303–313 [View Article][PubMed]
    [Google Scholar]
  12. Lafay B., Ruimy R., Rausch de Traubenberg C., Breittmayer V., Gauthier M. J., Christen R. 1995; Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . Int J Syst Bacteriol 45:290–296 [View Article][PubMed]
    [Google Scholar]
  13. Lyman J., Fleming R. H. 1940; Composition of seawater. J Mar Res 3:134–146
    [Google Scholar]
  14. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005; Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55:105–110 [View Article][PubMed]
    [Google Scholar]
  15. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T. 2006; Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . Int J Syst Evol Microbiol 56:1293–1304 [View Article][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Park J. R., Bae J. W., Nam Y.-D., Chang H.-W., Kwon H.-Y., Quan Z.-X., Park Y.-H. 2007; Sulfitobacter litoralis sp. nov., a marine bacterium isolated from the East Sea, Korea. Int J Syst Evol Microbiol 57:692–695 [View Article][PubMed]
    [Google Scholar]
  18. Pujalte M. J., Macián M. C., Arahal D. R., Garay E. 2005; Thalassobacter stenotrophicus Macián et al. 2005 is a later synonym of Jannaschia cystaugens Adachi et al. 2004, with emended description of the genus Thalassobacter . Int J Syst Evol Microbiol 55:1959–1963 [View Article][PubMed]
    [Google Scholar]
  19. Pukall R., Buntefuss D., Frühling A., Rohde M., Kroppenstedt R. M., Burghardt J., Lebaron P., Bernard L., Stackebrandt E. 1999; Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria . Int J Syst Bacteriol 49:513–519 [View Article][PubMed]
    [Google Scholar]
  20. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L. 1998; Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . Int J Syst Bacteriol 48:537–542 [View Article][PubMed]
    [Google Scholar]
  21. Sass H., Köpke B., Rütters H., Feuerlein T., Dröge S., Cypionka H., Engelen B. 2010; Tateyamaria pelophila sp. nov., a facultatively anaerobic alphaproteobacterium isolated from tidal-flat sediment, and emended descriptions of the genus Tateyamaria and of Tateyamaria omphalii . Int J Syst Evol Microbiol 60:1770–1777[PubMed] [CrossRef]
    [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  23. Schaefer J. K., Goodwin K. D., McDonald I. R., Murrell J. C., Oremland R. S. 2002; Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. Int J Syst Evol Microbiol 52:851–859 [View Article][PubMed]
    [Google Scholar]
  24. Shiba T. 1991; Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a . Syst Appl Microbiol 14:140–145 [View Article]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Sorokin D. Y. 1995; Sulfitobacter pontiacus gen. nov., sp. nov. – a new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. Microbiology (English translation of Mikrobiologiia) 64:295–305
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  29. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  30. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  31. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  32. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Mollica A., Faimali M., Vandamme P. 2009; Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 59:811–817 [View Article][PubMed]
    [Google Scholar]
  33. Wagner-Döbler I., Rheims H., Felske A., El-Ghezal A., Flade-Schröder D., Laatsch H., Lang S., Pukall R., Tindall B. J. 2004; Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites. Int J Syst Evol Microbiol 54:1177–1184 [View Article][PubMed]
    [Google Scholar]
  34. Yoon J. H., Kang S. J., Lee M. H., Oh T. K. 2007a; Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter . Int J Syst Evol Microbiol 57:1788–1792 [View Article]
    [Google Scholar]
  35. Yoon J. H., Kang S. J., Oh T. K. 2007b; Sulfitobacter marinus sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:302–305 [View Article][PubMed]
    [Google Scholar]
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