1887

Abstract

A Gram-stain-negative, rod-shaped bacterium (strain K-39) was isolated from the thermophilic phase of the composting process for oyster mushroom substrate preparation. The strain grew at 40–80 °C (optimum, 65–75 °C), at pH 5–9 (optimum, pH 7), in media containing up to 1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K-39 formed a distinct lineage within the genus . Its closest cultivated relative was PRI 3838 (96.8 % similarity). The DNA G+C content of strain K-39 was 71.3 mol%. The new strain could be differentiated from the related taxa by not being able to hydrolyse starch. The predominant fatty acids of strain K-39 were iso-C and anteiso-C. Strain K-39 contained a lower amount of the fatty acid iso-C as compared to related species of the genus . The predominant respiratory quinone of the new isolate was menaquinone MK-8. On the basis of a taxonomic study using a polyphasic approach, strain K-39 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is K-39 ( = DSM 21686 = NCAIM B 02340).

Funding
This study was supported by the:
  • Ministry of Agriculture and Rural Development (Hungary) (Award OMFB-00977/2005)
  • Ministry of Education and Culture, Hungary (Award DFÖ 0054/2009)
  • The European Union and the European Social Fund (Award TÁMOP 4.2.1./B-09/1/KMR-2010-0003)
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2012-07-01
2024-03-28
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