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Volume 62, Issue Pt_3

gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a haloalkaline lake Free

Abstract

Two bacterial isolates from the surface water of a haloalkaline lake on the Mongolia Plateau, strains MIM18 and MIM19, were characterized; their morphological, physiological and chemotaxonomic characters, and phylogenetic position based on 16S rRNA gene sequences, were determined. The two strains were alkaliphilic, halotolerant, non-motile, aerobic, Gram-negative, orange-red, rod-shaped and oxidase-positive. Growth was observed in 0–5.5 % (w/v) NaCl, with optimum growth at 1 %. The temperature range for growth was 0–41 °C, with good growth at 28–37 °C and optimum growth at 30–33 °C. The DNA G+C content was 39.8–41.2 mol%. The strains contained menaquinone MK-7 as the major respiratory quinone and phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Predominant cellular fatty acids were iso-C (28.1–29.3 %), iso-C 3-OH (14.6–18.8 %), Cω6 (5.3–8.6 %), C 2-OH and/or iso-C 2-OH (5.4–6.1 %), and iso-Cω9 and/or C 10-methyl (5.0–6.8 %). 16S rRNA gene sequence analysis showed that and of the family were the closest related species with sequence similarities of 91.7–92.3 % and 88.2 %, respectively, with strains of these genera; other members of the had sequence similarities lower than 88 %. Phylogenetic analysis indicated that the strains formed a deep-rooted lineage distinct from the clades represented by the genera , , , , , , and . Based on the phenotypic and phylogenetic characteristics mentioned above, the two strains are representatives of a single novel species in a new genus; the name gen. nov., sp. nov. is proposed, with MIM18 ( = ACCC 05421 = KCTC 23224) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30860015 and 30760004)
  • Major Program of Natural Science Foundation of Inner Mongolia (Award K32715 and 2009ZD03)
  • Innovative Research Group of Inner Mongolia Agriculture University (Award NDPYTD2010-3)
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2012-03-01
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Mongoliitalea lutea gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a haloalkaline lake
Int J Syst Evol Microbiol 62, 647 (2012); https://doi.org/10.1099/ijs.0.031286-0
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