%0 Journal Article %A Männistö, Minna K. %A Rawat, Suman %A Starovoytov, Valentin %A Häggblom, Max M. %T Granulicella arctica sp. nov., Granulicella mallensis sp. nov., Granulicella tundricola sp. nov. and Granulicella sapmiensis sp. nov., novel acidobacteria from tundra soil %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_9 %P 2097-2106 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.031864-0 %I Microbiology Society, %X Four aerobic bacteria, designated MP5ACTX2T, MP5ACTX8T, MP5ACTX9T and S6CTX5AT, were isolated from tundra soil of north-western Finland (69° 03′ N 20° 50′ E). Cells of all isolates were Gram-negative, non-motile rods. Phylogenetic analysis indicated that they belonged to the genus Granulicella of subdivision 1 of the phylum Acidobacteria . 16S rRNA gene sequence similarity between the new isolates and the type strains of Granulicella aggregans , Granulicella paludicola , Granulicella pectinivorans and Granulicella rosea ranged from 94 to 99 %. Analysis of the RNA polymerase beta subunit (rpoB) gene sequence indicated that the isolates represented novel species of the genus Granulicella (<92 % rpoB sequence similarity between the isolates and members of the genus Granulicella ). This was also confirmed by low DNA–DNA relatedness (31 %) between strain S6CTX5AT and the type strain of G. pectinivorans , which exhibited 99.1 % 16S rRNA gene sequence similarity and 91.7 % rpoB gene sequence similarity. The isolates grew at pH 3.5–6.5 and at 4–26 °C. Sugars were the preferred growth substrates. The major cellular fatty acids were iso-C15 : 0, C16 : 1ω7c and C16 : 0 and the major isoprenoid quinone was MK-8. The DNA G+C content was 56–60 mol%. On the basis of phylogenetic analysis and chemotaxonomic and physiological data, the isolates represent four novel species of the genus Granulicella , for which the names Granulicella arctica MP5ACTX2T ( = ATCC BAA-1858T = DSM 23128T), Granulicella mallensis MP5ACTX8T ( = ATCC BAA-1857T = DSM 23137T), Granulicella tundricola MP5ACTX9T (ATCC BAA-1859T = DSM 23138T) and Granulicella sapmiensis S6CTX5AT ( = LMG 26174T = DSM 23136T) are proposed. An emended description of the genus Granulicella is also presented. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.031864-0