1887

Abstract

A Gram-positive, rod-shaped, xylanolytic, spore-forming bacterium, strain GTH-3, was isolated from a tidal flat adjacent to Ganghwa Island, Republic of Korea, and was characterized to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain GTH-3 was shown to belong to the family , being most closely related to the type strains of (94.9 %), (94.8 %), (94.4 %), (94.2 %), (94.1 %) and (94.0 %). The G+C content of the genomic DNA of strain GTH-3 was 45.9±0.2 mol% (mean±). The major menaquinone was MK-7. The major fatty acids were anteiso-C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain GTH-3 to the genus . The results of physiological and biochemical tests allowed strain GTH-3 to be distinguished genotypically and phenotypically from recognized species of the genus . Strain GTH-3 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GTH-3 ( = DSM 23491  = LMG 25635).

Funding
This study was supported by the:
  • 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science and Technology (Award MG08-0101-2-0)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032102-0
2012-06-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/6/1284.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032102-0&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260[PubMed] [CrossRef]
    [Google Scholar]
  2. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  4. Coughlan M. P., Hazlewood G. P. 1993; β-1,4-d-xylan-degrading enzyme systems: biochemistry, molecular biology and applications. Biotechnol Appl Biochem 17:259–289[PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  7. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
    [Google Scholar]
  8. Kim M. K., Im W.-T., Ohta H., Lee M., Lee S.-T. 2005; Sphingopyxis granuli sp. nov., a β-glucosidase-producing bacterium in the family Sphingomonadaceae in α-4 subclass of the Proteobacteria . J Microbiol 43:152–157[PubMed]
    [Google Scholar]
  9. Kim K. K., Lee K. C., Lee J.-S. 2011; Reclassification of Paenibacillus ginsengisoli as a later heterotypic synonym of Paenibacillus anaericanus . Int J Syst Evol Microbiol 61:2101–2106 [View Article][PubMed]
    [Google Scholar]
  10. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  11. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163[PubMed] [CrossRef]
    [Google Scholar]
  12. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. other authors 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121[PubMed] [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  15. Moore D. D., Dowhan D. 1995; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp. 2–11 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc..
    [Google Scholar]
  18. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298[PubMed] [CrossRef]
    [Google Scholar]
  19. Ten L. N., Im W.-T., Kim M.-K., Kang M.-S., Lee S.-T. 2004; Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56:375–382[PubMed] [CrossRef]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[PubMed] [CrossRef]
    [Google Scholar]
  21. Timmell T. E. 1967; Recent progress in the chemistry of wood hemicelluloses. Wood Sci Technol 1:45–70 [CrossRef]
    [Google Scholar]
  22. Wang L., An D.-S., Jin F.-X., Lee S.-T., Im W.-T., Bae H.-M. 2011; Phycicoccus ginsenosidimutans sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 61:524–528[PubMed] [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032102-0
Loading
/content/journal/ijsem/10.1099/ijs.0.032102-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error