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Abstract
Three isolates of Gram-negative, rod-shaped, non-spore-forming bacteria were recovered from galls on chrysanthemum (Chrysanthemum L.; Ch11T, Ch12) and cherry plum (Prunus cerasifera var. divaricata; AL9.3). All three isolates were able to cause crown galls on various plant species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the three isolates were probably identical (100% sequence similarity) and closely related to Rhizobium rubi (99.6 %), Rhizobium radiobacter (98.7 %) and Rhizobium larrymoorei (98.1 %). Similar analysis based on the housekeeping genes glnA, gyrB and rpoB also indicated that the novel isolates were identical and closely related to R. rubi . The major cellular fatty acids of strain Ch11T were C18 : 1ω7c (62.1 %), summed feature 2 (comprising C12 : 0 aldehyde, iso-C16 : 1 I and/or C14 : 0 3-OH; 10.8 %), summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH; 7.7 %) and C10 : 0 3-OH (7.5 %). However, the DNA–DNA relatedness between Ch11T and R. rubi LMG 156T was only 48 % and, unlike phylogenetically related established Rhizobium species, the novel isolates were able to utilize β-hydroxybutyric acid but not l-fucose. Based on the phylogenetic and phenotypic evidence, the isolates are considered to represent a single novel species of the genus Rhizobium , for which the name Rhizobium skierniewicense sp. nov. is proposed; the type strain is Ch11T ( = LMG 26191T = CFBP 7420T).
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Funding
- Polish Scientific Committee (Award 118/N-COST/2008/0)
- Fund for Scientific Research-Flanders (Award G.0058.07N)