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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_7

sp. nov., a thermophilic, ethanol-producing bacterium isolated from a hot spring Free

Abstract

A novel moderately thermophilic, anaerobic, ethanol-producing bacterial strain, 45B, was isolated from a mixed sediment water sample collected from a hot spring at Potosi, Bolivia. The cells were straight to slightly curved rods approximately 2.5 µm long and 0.5 µm wide. The strain was Gram-stain-variable, spore-forming and monotrichously flagellated. Growth of the strain was observed at 45–65 °C and pH 5.5–8.0, with optima of 60 °C and pH 6.5. The substrates utilized by strain 45B were xylose, cellobiose, glucose, arabinose, sucrose, lactose, maltose, fructose, galactose, mannose, glycerol, xylan, carboxymethylcellulose and yeast extract. The main fermentation product from xylose and cellobiose was ethanol (0.70 and 0.45 g ethanol per gram of consumed sugar, respectively). Acetate, lactate, propionate, carbon dioxide and hydrogen were also produced in minor quantities. 1,3-Propanediol was produced when glycerol-containing medium was supplemented with yeast extract. The major cellular fatty acids were anteiso-C, C, iso-C, C, iso-C, C and C. The polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an aminoglycolipid and 15 other unidentified lipids were predominant. The DNA G+C content of strain 45B was 32.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain 45B is located within the Gram-type positive branch of the phylogenetic tree. On the basis of morphological and physiological properties and phylogenetic analysis, strain 45B represents a novel species, for which the name sp. nov. is proposed; the type strain is 45B ( = DSM 22065 = CCUG 57396).

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Funding
This study was supported by the:
  • Swedish International Development Cooperation Agency
  • Swedish Agency for Research Cooperation with Developing Countries
© 2012 IUMS
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2012-07-01
2024-04-23
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Caloramator boliviensis sp. nov., a thermophilic, ethanol-producing bacterium isolated from a hot spring
Int J Syst Evol Microbiol 62, 1679 (2012); https://doi.org/10.1099/ijs.0.032664-0
/content/journal/ijsem/10.1099/ijs.0.032664-0
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