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Volume 62, Issue 2

sp. nov., from a marine solar saltern Free

Abstract

A Gram-staining-variable, motile, endospore-forming and rod-shaped bacterial strain, IDS-20, was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic investigation. Strain IDS-20 grew optimally at 37 °C, at pH 7.5–8.0 and in the presence of 4–5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IDS-20 belongs to the genus . Strain IDS-20 exhibited 93.4–96.6 % 16S rRNA gene sequence similarity to the type strains of species of the genus . Strain IDS-20 had MK-7 as the predominant menaquinone and a cell-wall peptidoglycan based on -diaminopimelic acid. The major fatty acids were anteiso-C and anteiso-C and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 39.5 mol%. The phylogenetic distinctiveness and differential phenotypic characteristics of strain IDS-20 demonstrated that this strain can be distinguished from recognized species of the genus . On the basis of the data presented, strain IDS-20 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IDS-20 ( = KCTC 13727  = CCUG 59308).

  • Published Online:
Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
  • 21C Frontier Program of Microbial Genomics and Applications (Award MG05-0401-2-0)
  • Ministry of Education, Science and Technology (MEST) of the Republic of Korea
© 2012 IUMS
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2012-02-01
2024-04-20
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References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes . In The Prokaryotes vol. 2 pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  3. Carrasco I. J., Márquez M. C., Ventosa A. 2009; Virgibacillus salinus sp. nov., a moderately halophilic bacterium from sediment of a saline lake. Int J Syst Evol Microbiol 59:3068–3073 [View Article][PubMed]
     [Google Scholar]
  4. Chen Y.-G., Cui X.-L., Fritze D., Chai L.-H., Schumann P., Wen M.-L., Wang Y.-X., Xu L.-H., Jiang C.-L. 2008; Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 58:647–653 [View Article][PubMed]
     [Google Scholar]
  5. Chen Y.-G., Cui X.-L., Wang Y.-X., Zhang Y.-Q., Tang S.-K., Li W.-J., Liu Z.-X., Wen M.-L., Peng Q. 2009; Virgibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 59:2058–2063 [View Article][PubMed]
     [Google Scholar]
  6. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [View Article]
     [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  8. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. 1998; Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . Int J Syst Bacteriol 48:99–106 [View Article]
     [Google Scholar]
  9. Heyrman J., Logan N. A., Busse H.-J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. 2003; Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53:501–511 [View Article][PubMed]
     [Google Scholar]
  10. Hua N.-P., Hamza-Chaffai A., Vreeland R. H., Isoda H., Naganuma T. 2008; Virgibacillus salarius sp. nov., a halophilic bacterium isolated from a Saharan salt lake. Int J Syst Evol Microbiol 58:2409–2414 [View Article][PubMed]
     [Google Scholar]
  11. Jeon C. O., Kim J. M., Park D.-J., Xu L.-H., Jiang C.-L., Kim C.-J. 2009; Virgibacillus xinjiangensis sp. nov., isolated from a Salt Lake of Xin-jiang Province in China. J Microbiol 47:705–709 [View Article][PubMed]
     [Google Scholar]
  12. Jeon C. O., Kim J. M., Park D.-J., Xu L.-H., Jiang C.-L., Kim C.-J. 2010; Virgibacillus xinjiangensis sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 133. Int J Syst Evol Microbiol 60:1009–1010 [View Article][PubMed]
     [Google Scholar]
  13. Kämpfer P., Arun A. B., Busse H.-J., Langer S., Young C.-C., Chen W.-M., Syed A. A., Rekha P. D. 2011; Virgibacillus soli sp. nov., isolated from mountain soil. Int J Syst Evol Microbiol 61:275–280 [View Article][PubMed]
     [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  15. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  16. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
     [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  18. Niederberger T. D., Steven B., Charvet S., Barbier B., Whyte L. G. 2009; Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic. Int J Syst Evol Microbiol 59:2219–2225 [View Article][PubMed]
     [Google Scholar]
  19. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  20. Sehgal S. N., Gibbons N. E. 1960; Effect of some metal ions on the growth of Halobacterium cutirubrum . Can J Microbiol 6:165–169 [View Article][PubMed]
     [Google Scholar]
  21. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
     [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  23. Wang C.-Y., Chang C.-C., Ng C. C., Chen T.-W., Shyu Y.-T. 2008; Virgibacillus chiguensis sp. nov., a novel halophilic bacterium isolated from Chigu, a previously commercial saltern located in southern Taiwan. Int J Syst Evol Microbiol 58:341–345 [View Article][PubMed]
     [Google Scholar]
  24. Wang X., Xue Y., Ma Y. 2010; Virgibacillus subterraneus sp. nov., a moderately halophilic Gram-positive bacterium isolated from subsurface saline soil. Int J Syst Evol Microbiol 60:2763–2767 [View Article][PubMed]
     [Google Scholar]
  25. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
     [Google Scholar]
  26. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
     [Google Scholar]
  27. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
     [Google Scholar]
  28. Yoon J.-H., Oh T.-K., Park Y.-H. 2004; Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54:2163–2167 [View Article][PubMed]
     [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Jung Y.-T., Lee K. C., Oh H. W., Oh T.-K. 2010; Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 60:291–295 [View Article][PubMed]
     [Google Scholar]
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Virgibacillus campisalis sp. nov., from a marine solar saltern
Int J Syst Evol Microbiol 62, 347 (2012); https://doi.org/10.1099/ijs.0.033084-0
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