1887

Abstract

Among ballistoconidium-forming yeast strains isolated from various plant leaves collected from subtropical forests in eastern and central China, four strains forming cream to yellowish coloured colonies were revealed to represent three novel species by conventional and molecular characterization. Phylogenetic analysis based on combined sequences of the internal transcribed spacer (ITS) and 26S rRNA gene D1/D2 domain showed that strains GT-753 and ZJJ-890 were conspecific and closely related to , and . Strain ZJJ-394 was basal to the branch formed by , and with strong bootstrap support. Strain GT-475 was closely related to . The strains differed significantly from their close relatives in D1/D2 and ITS sequences and in physiological criteria. Three novel species are proposed: sp. nov. (type strain ZJJ-890 = CGMCC 2.4407 = CBS 12233), sp. nov. (type strain GT-475 = CGMCC 2.4411 = CBS 12234) and sp. nov. (type strain ZJJ-394 = CGMCC 2.4409 = CBS 12259).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31010103902 and 30825002)
  • Royal Netherlands Academy of Arts and Sciences (Award 10CDP019)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.033241-0
2012-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/4/996.html?itemId=/content/journal/ijsem/10.1099/ijs.0.033241-0&mimeType=html&fmt=ahah

References

  1. Bai F.-Y., Takashima M., Nakase T. 2001; Phylogenetic analysis of strains originally assigned to Bullera variabilis: descriptions of Bullera pseudohuiaensis sp. nov., Bullera komagatae sp. nov. and Bullera pseudoschimicola sp. nov.. Int J Syst Evol Microbiol 51:2177–2187 [View Article][PubMed]
    [Google Scholar]
  2. Bai F.-Y., Zhao J.-H., Takashima M., Jia J.-H., Boekhout T., Nakase T. 2002; Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov.. Int J Syst Evol Microbiol 52:2309–2314 [View Article][PubMed]
    [Google Scholar]
  3. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Fungsin B., Takashima M., Sugita T., Artjariyasripong S., Potacharoen W., Tanticharoen M., Nakase T. 2006; Bullera koratensis sp. nov. and Bullera lagerstroemiae sp. nov., two new ballistoconidium-forming yeast species in the Trichosporonales clade isolated from plant leaves in Thailand. J Gen Appl Microbiol 52:73–81 [View Article][PubMed]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  7. Makimura K., Murayama S. Y., Yamaguchi H. 1994; Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 40:358–364 [View Article][PubMed]
    [Google Scholar]
  8. Nakase T., Takashima M. 1993; A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Rev 3:33–34
    [Google Scholar]
  9. Nakase T., Tsuzuki S., Takashima M. 2002; Bullera taiwanensis sp nov and Bullera formosensis sp. nov., two new ballistoconidium-forming yeast species isolated from plant leaves in Taiwan. J Gen Appl Microbiol 48:345–355 [View Article][PubMed]
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  11. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. 2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEM Yeast Res 2:495–517[PubMed] [CrossRef]
    [Google Scholar]
  12. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  13. Wang Q.-M., Bai F.-Y. 2008; Molecular phylogeny of basidiomycetous yeasts in the Cryptococcus luteolus lineage (Tremellales) based on nuclear rRNA and mitochondrial cytochrome b gene sequence analysis: proposal of Derxomyces gen. nov. and Hannaella gen. nov., and description of eight novel Derxomyces species. FEM Yeast Res 8:799–814 [View Article]
    [Google Scholar]
  14. Wang Q.-M., Bai F.-Y., Lu H.-Z., Jia J.-H., Takashima M. 2004; Bullera cylindrica sp. nov., Bullera hubeiensis sp. nov. and Bullera nakasei sp. nov., ballistoconidium-forming yeast species from plant leaves. Int J Syst Evol Microbiol 54:1877–1882 [View Article][PubMed]
    [Google Scholar]
  15. Wang Q.-M., Bai F.-Y., Fungsin B., Boekhout T., Nakase T. 2011; Proposal of Mingxiaea gen. nov. for the anamorphic basidiomycetous yeast species in the Bulleribasidium clade (Tremellales) based on molecular phylogenetic analysis, with six new combinations and four novel species. Int J Syst Evol Microbiol 61:210–219 [View Article][PubMed]
    [Google Scholar]
  16. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp. 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.033241-0
Loading
/content/journal/ijsem/10.1099/ijs.0.033241-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error