Deinococcus daejeonensis sp. nov., isolated from sludge in a sewage disposal plant
Srinivasan, Sathiyaraj and Kim, Myung Kyum and Lim, Sangyong and Joe, Minho and Lee, Myungjin,, 62, 1265-1270 (2012),
doi = https://doi.org/10.1099/ijs.0.033316-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-positive, strictly aerobic, spherical, non-motile red-pigmented bacterial strain, designated MJ27T, was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was used to study the taxonomic position of strain MJ27T. Strain MJ27T shared highest 16S rRNA gene sequence similarity with Deinococcus grandis DSM 3963T (98.8 %), Deinococcus caeni Ho-08T (97.5 %) and Deinococcus aquaticus PB314T (96.6 %.); levels of sequence similarity with the type strains of other Deinococcus species were less than 96.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ27T belonged to the clade formed by members of the genus Deinococcus in the family Deinococcaceae . The G+C content of the genomic DNA of strain MJ27T was 67.6 mol%. The chemotaxonomic characteristics of strain MJ27T were typical of members of the genus Deinococcus , with MK-8 as the predominant respiratory quinone, C16 : 1ω7c, C15 : 1ω6c, C16 : 0 and C15 : 0 as major fatty acids (>12 %), ornithine as the diamino acid in the cell-wall peptidoglycan and resistance to gamma radiation [D10 (dose required to reduce the bacterial population by tenfold) >9 kGy]. The low levels of DNA–DNA relatedness reported here (5.3±1.5–29.2±2.3 %) indicate that strain MJ27T represents a species that is separate from its closest relatives in the genus Deinococcus . On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ27T is considered to represent a novel species of the genus Deinococcus , for which the name Deinococcus daejeonensis sp. nov. is proposed. The type strain is MJ27T ( = KCTC 13751T = JCM 16918T).,
language=,
type=