Alteribacillus bidgolensis gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Bacillus persepolensis as Alteribacillus persepolensis comb. nov.
Didari, Maryam and Amoozegar, Mohammad Ali and Bagheri, Maryam and Schumann, Peter and Spröer, Cathrin and Sánchez-Porro, Cristina and Ventosa, Antonio,, 62, 2691-2697 (2012),
doi = https://doi.org/10.1099/ijs.0.034173-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A novel Gram-stain-positive, moderately halophilic bacterium, designated strain P4BT, was isolated from water of the hypersaline Aran-Bidgol lake in Iran and characterized taxonomically by using a polyphasic approach. Cells of strain P4BT were non-motile rods producing ellipsoidal endospores at a central position in non-swollen sporangia. Strain P4BT was strictly aerobic and catalase- and oxidase-positive. It was able to grow at NaCl concentrations of 0.5–12.5 % (w/v), with optimum growth occurring at 5–7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain P4BT was shown to belong to the phylum Firmicutes and shared highest similarity with Bacillus persepolensis HS136T (97.1 %) and Bacillus salarius BH169T (95.1 %). However, it shared only 91.3 % 16S rRNA gene sequence similarity with Bacillus subtilis subsp. subtilis DSM 10T, indicating that strain P4BT might not be a member of the genus Bacillus . The DNA G+C content of this new isolate was 38.9 mol%. DNA–DNA hybridization experiments revealed a low level of relatedness between strain P4BT and B. persepolensis HS136T (6 %). The major cellular fatty acids of strain P4BT were iso-C15 : 0 and anteiso-C15 : 0, as for B. persepolensis HS136T but in contrast to B. salarius DSM 16461T and B. subtilis subsp. subtilis DSM 10T. Its polar lipid pattern consisted of phosphatidylglycerol, an aminoglycolipid and an unknown phospholipid. This polar lipid profile was similar to that obtained for B. persepolensis DSM 21632T but different from those of B. salarius DSM 16461T and B. subtilis subsp. subtilis DSM 10T. The isoprenoid quinones were MK-7 (88 %) and MK-8 (2 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features indicate placement of strain P4BT within the Firmicutes , closely related to B. persepolensis but with features clearly distinct from those of the genus Bacillus and other related genera. On the basis of these data, strain P4BT is considered to represent a novel species of a new genus, for which the name Alteribacillus bidgolensis gen. nov., sp. nov. is proposed. The type strain of Alteribacillus bidgolensis is P4BT ( = CCM 7963T = CECT 7998T = DSM 25260T = IBRC-M 10614T = KCTC 13821T). It is also suggested to transfer B. persepolensis to this new genus, as Alteribacillus persepolensis comb. nov. The type strain of Alteribacillus persepolensis is HS136T ( = CCM 7595T = DSM 21632T = JCM 15720T = LMG 25222T).,
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