1887

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Volume 62, Issue Pt_8

sp. nov., a thermotolerant actinomycete isolated from soil, and emended description of the genus Free

Abstract

A strain of thermotolerant actinomycete, designated YIM 75948, was isolated from a soil sample in Yunnan province, China. The strain grew at 25–55 °C (optimum 37 °C). The substrate mycelium and aerial mycelium produced on Czapek’s agar were both pale yellow to white. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid, and the whole-cell sugars were mannose, ribose, glucose, galactose and arabinose. The major fatty acids were iso-C, iso-C and C and the predominant respiratory quinone was MK-9(H) The polar lipids consisted of phosphatidylethanolamine, phosphatidylethanolamine with hydroxy fatty acids, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. The genomic DNA G+C content was 73.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 75948 belonged in the genus and that its closest relative among recognized species was DSM 43855 (97.6 % sequence similarity). The mean level of DNA–DNA relatedness between the novel strain and DSM 43855 was, however, only 47.8 %. Based on the phenotypic, chemotaxonomic and phylogenetic data and the results of the DNA–DNA hybridizations, strain YIM 75948 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is YIM 75948 ( = JCM 17695 = DSM 45613). The description of the genus is emended to reflect the fact that the genomic DNA G+C contents of DSM 43855 and the type strain of sp. nov. recorded in the present study fell above the range given in previous descriptions of this genus.

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Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award 31070007 and 31000001)
  • China Postdoctoral Science Foundation (Award 20090461410)
© 2012 IUMS
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2012-08-01
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Actinokineospora soli sp. nov., a thermotolerant actinomycete isolated from soil, and emended description of the genus Actinokineospora
Int J Syst Evol Microbiol 62, 1845 (2012); https://doi.org/10.1099/ijs.0.035832-0
/content/journal/ijsem/10.1099/ijs.0.035832-0
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