@article{mbs:/content/journal/ijsem/10.1099/ijs.0.036160-0, author = "Schumann, Peter and Zhang, De-Chao and Redzic, Mersiha and Margesin, Rosa", title = "Alpinimonas psychrophila gen. nov., sp. nov., an actinobacterium of the family Microbacteriaceae isolated from alpine glacier cryoconite", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2012", volume = "62", number = "Pt_11", pages = "2724-2730", doi = "https://doi.org/10.1099/ijs.0.036160-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.036160-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-type positive, Gram-reaction variable, non-motile, psychrophilic actinobacterium, designated Cr8-25T, was isolated from alpine glacier cryoconite and was able to grow well over a temperature range of 1–15 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Cr8-25T belonged to the family Microbacteriaceae and showed highest 16S rRNA gene sequence similarity with Klugiella xanthotipulae 44C3T (97.0 %). However, strain Cr8-25T could be differentiated from the type strain of K. xanthotipulae on the level of genomospecies by a DNA–DNA relatedness value of only 37.2 %. Strain Cr8-25T contained a cell-wall peptidoglycan that was cross-linked according to the B-type, which is based on 2,4-diaminobutyric acid. The cell wall contained the sugars galactose, fucose and rhamnose. The predominant cellular fatty acids of strain Cr8-25T were C15 : 0 anteiso (64.6 %) and iso-C16 : 0 (22.5 %) and the major menaquinones were MK-11 and MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and unknown glycolipids. The G+C content of the genomic DNA was 58.8 mol%. On the basis of the phenotypic characteristics, phylogenetic and chemotaxonomic analyses and DNA–DNA relatedness data, strain Cr8-25T represents a novel species of a new genus in the family Microbacteriaceae , for which the name Alpinimonas psychrophila gen. nov., sp. nov. is proposed. The type strain is Cr8-25T ( = DSM 23737T = LMG 26215T).", }