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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_10

gen. nov., sp. nov., a new member of the family Free

Abstract

A novel Gram-positive, aerobic, spore-forming, thermophilic actinomycete, designated strain YIM 77521, was isolated from a sandy soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. The strain formed branched substrate mycelia and no fragmentation was found. Masses of short, straight or irregular chains of three to eight warty ornamented spores were borne from aerial mycelia. The strain contained -diaminopimelic acid in the cell wall and the whole-cell sugars contained mannose, galactose, glucose and ribose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylmonomethylethanolamine and -acetylglucosamine-containing phospholipids. The major fatty acids were iso-C, C 10-methyl and C. The DNA G+C content of strain YIM 77521 was 73.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 77521 fell within the radiation of the suborder and formed a distinct monophyletic lineage adjacent to the family with a high bootstrap value. On the basis of combined data from the phenotypic and phylogenetic analyses, strain YIM 77521 represents a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is YIM 77521 ( = DSM 45615  = CCTCC AA 2011013).

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Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award 31070007)
  • Foundation of Yunnan University (Award 2009C16Q)
© 2012 IUMS
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2012-10-01
2024-04-20
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References

  1. Ara I., Kudo T. 2007; Sphaerosporangium gen. nov., a new member of the family Streptosporangiaceae, with descriptions of three new species as Sphaerosporangium melleum sp. nov., Sphaerosporangium rubeum sp. nov. and Sphaerosporangium cinnabarinum sp. nov., and transfer of Streptosporangium viridialbum Nonomura and Ohara 1960 to Sphaerosporangium viridialbum comb. nov.. Actinomycetologica 21:11–21 [View Article]
     [Google Scholar]
  2. Cao Y. R., Jiang Y., Xu L. H., Jiang C. L. 2009; Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China. Int J Syst Evol Microbiol 59:1679–1684 [View Article][PubMed]
     [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  4. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [View Article]
     [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
     [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  9. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  11. Goodfellow M., Stanton L. J., Simpson K. E., Minnikin D. E. 1990; Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136:19–36 [View Article]
     [Google Scholar]
  12. Goodfellow M., Maldonado L. A., Quintana E. T. 2005; Reclassification of Nonomuraea flexuosa (Meyer 1989) Zhang et al. 1998 as Thermopolyspora flexuosa gen. nov., comb. nov., nom. rev.. Int J Syst Evol Microbiol 55:1979–1983 [View Article][PubMed]
     [Google Scholar]
  13. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
     [Google Scholar]
  14. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [View Article]
     [Google Scholar]
  15. Greiner-Mai E., Kroppenstedt R. M., Korn-Wendisch F., Kutzner H. J. 1987; Morphological and biochemical characterization and emended descriptions of thermophilic actinomycete species. Syst Appl Microbiol 9:97–109 [View Article]
     [Google Scholar]
  16. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
     [Google Scholar]
  17. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
     [Google Scholar]
  18. Kelly K. L. 1964 Inter-Society Color Council - National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
     [Google Scholar]
  19. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  20. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series no. 20) pp. 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  21. Kudo T., Itoh T., Miyadoh S., Shomura T., Seino A. 1993; Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol 43:319–328 [View Article][PubMed]
     [Google Scholar]
  22. Lechevalier H. A., Lechevalier M. P. 1970a; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales pp. 393–405 Edited by Prauser H. Jena: VEB Gustav Fischer;
     [Google Scholar]
  23. Lechevalier M. P., Lechevalier H. A. 1970b; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
     [Google Scholar]
  24. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [View Article]
     [Google Scholar]
  25. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
     [Google Scholar]
  26. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  27. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [View Article]
     [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  29. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
     [Google Scholar]
  30. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  31. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:479–491 [View Article]
     [Google Scholar]
  32. Stackebrandt E., Wink J., Steiner U., Kroppenstedt R. M. 2001; Nonomuraea dietzii sp. nov.. Int J Syst Evol Microbiol 51:1437–1441[PubMed]
     [Google Scholar]
  33. Tamura T., Sakane T. 2004; Planotetraspora silvatica sp. nov. and emended description of the genus Planotetraspora . Int J Syst Evol Microbiol 54:2053–2056 [View Article][PubMed]
     [Google Scholar]
  34. Tamura T., Suzuki S., Hatano K. 2000; Acrocarpospora gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:1163–1171 [View Article][PubMed]
     [Google Scholar]
  35. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  36. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J. 2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59:2025–2031 [View Article][PubMed]
     [Google Scholar]
  37. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  38. Waksman S. A. 1961 The Actinomycetes. Classification, Identification and Description of Genera and Species vol. 2 Baltimore: The Williams & Wilkins Co;
     [Google Scholar]
  39. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
     [Google Scholar]
  40. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
     [Google Scholar]
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Thermocatellispora tengchongensis gen. nov., sp. nov., a new member of the family Streptosporangiaceae
Int J Syst Evol Microbiol 62, 2417 (2012); https://doi.org/10.1099/ijs.0.036897-0
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