RT Journal Article SR Electronic(1) A1 Zhang, Wei-Yan A1 Fang, Ming-Xu A1 Zhang, Wen-Wu A1 Xiao, Chuan A1 Zhang, Xin-Qi A1 Yu, Zhi-Ping A1 Zhu, Xu-Fen A1 Wu, MinYR 2013 T1 Extensimonas vulgaris gen. nov., sp. nov., a member of the family Comamonadaceae JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_6 SP 2062 OP 2068 DO https://doi.org/10.1099/ijs.0.038158-0 PB Microbiology Society, SN 1466-5034, AB A novel strain, named S4T, was obtained from industrial wastewater in Xiaoshan, Zhejiang Province, China. Cells were Gram-negative, neutrophilic and non-spore-forming and moved by means of a polar flagellum. Normal cells were 0.8–0.9×1.3–1.9 µm and the cells elongated to 10–25 µm when cultivated at high temperatures. Strain S4T grew at 15–50 °C (optimum at 48 °C), pH 5.5–8.5 (optimum 7.0–7.5) and 0–2 % (optimum 0.5 %) (w/v) NaCl. Ubiquinone-8 was the predominant respiratory quinone. C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C17 : 0 cyclo were the major cellular fatty acids. The major 3-OH fatty acid was C10 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminoglycolipid. The genomic DNA G+C content was 68.8 mol%. Based on 16S rRNA gene sequences alignment, the most closely related strains were members of the genera Comamonas (94.6–95.6 % similarities), Giesbergeria (94.9–95.6 %), Acidovorax (94.8–95.4 %), Brachymonas (94.1–95.2 %) and Macromonas (95.1 %). Phylogenetic analysis showed the closest relatives of strain S4T were members of the genus Macromonas . Based on phenotypic and phylogenetic characteristics, we suggest that strain S4T represents a novel species of a new genus of the family Comamonadaceae , for which the name Extensimonas vulgaris gen. nov., sp. nov. is proposed. The type strain of Extensimonas vulgaris is S4T ( = CGMCC 1.10977T = JCM 17803T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.038158-0