Exiguobacterium alkaliphilum sp. nov. isolated from alkaline wastewater drained sludge of a beverage factory
Mohan Kulshreshtha, Niha and Kumar, Rita and Begum, Zareena and Shivaji, S. and Kumar, Anil,, 63, 4374-4379 (2013),
doi = https://doi.org/10.1099/ijs.0.039123-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A facultatively anaerobic, alkaliphilic, Gram-stain-positive, rod-shaped bacterium, designated strain 12/1T, isolated from alkaline wastewater drained sludge of a beverage industry facility located near New Delhi, India, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain 12/1T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium aurantiacum DSM 6208T (99.46 %), E. aquaticum IMTB-3094T (99.18 %), E. mexicanum 8NT (99.06 %), E. profundum 10CT (98.17 %), E. aestuarii TF-16T (98.1 %) and E. marinum TF-80T (98.03 %). The DNA G+C content of strain 12/1T was 55.6 mol%, major respiratory isoprenoid quinone was MK-7, major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine and the cell-wall peptidoglycan was of the A3α l-Lys–Gly type, characteristics consistent with its affiliation to the genus Exiguobacterium . Strain 12/1T showed levels of DNA–DNA hybridization of less than 70 % with the closely related species of the genus Exiguobacterium . Overall, the phenotypic, chemotaxonomic and phylogenetic data presented in this study suggest that strain 12/1T represents a novel species of the genus Exiguobacterium , for which the name Exiguobacterium alkaliphilum sp. nov. is proposed. The type strain is 12/1T ( = CCM 8459T = DSM 21148T).,
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