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Volume 63, Issue Pt_2

gen. nov., sp. nov., a member of the phylum isolated from soil Free

Abstract

An aerobic chemoheterotrophic gliding bacterium, designated RYG, was isolated from a soil in Germany. Cells were Gram-stain-negative, thin rods (0.4–0.6 µm in width and 2.0–5.5 µm in length). Cells multiplied by normal cell division and no resting stages were observed. Colonies were yellow and displayed swarming edges. Gliding motility was observed in wet mounts. Strain RYG grew at pH 5.6–7.7 (optimum pH 6.6–7.0), at 13–37 °C (optimum 25–30 °C) and with 0–1.0 % NaCl (optimum 0–0.1 %). The isolate was incapable of atmospheric nitrogen fixation and grew on most mono- and disaccharides as well as a few polysaccharides and organic acids. The predominant menaquinone was MK-7, the major cellular fatty acids were Cω5 and iso-C and the major intact polar lipids were composed of phosphatidylethanolamine derivatives and two unknown series. The DNA G+C content was 49.9 mol%. Based on 16S rRNA gene sequence analysis, the isolate belonged to the phylum , class , order , but was only distantly related to any cultured bacteria. The closest relatives were 3B-2 and 10AO (both 93 % 16S rRNA gene sequence similarity). We propose a novel genus and species, gen. nov., sp. nov.. Strain RYG ( = DSM 24574 = ATCC BAA-2075) is the type strain.

  • Published Online:
Funding
This study was supported by the:
  • Max-Planck-Society
  • Natural Sciences and Engineering Research Council of Canada (NSERC)
© 2013 IUMS
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2013-02-01
2024-04-19
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Chryseolinea serpens gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from soil
Int J Syst Evol Microbiol 63, 654 (2013); https://doi.org/10.1099/ijs.0.039404-0
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