1887

Abstract

A borrelidin-producing actinomycete, designated strain NEAU-W2, was isolated from the root surface of soybean [ (L.) Merr] and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. The G+C content of the DNA was 66.12 mol%. Analysis of the 16S rRNA gene sequence of strain NEAU-W2 revealed that the strain formed a distinct clade within the 16S rRNA gene sequence phylogenetic tree and showed highest similarity (99.61 %) to ATCC 27449. However, the DNA–DNA relatedness between strain NEAU-W2 and ATCC 27449 was 58.51 %. Strain NEAU-W2 could also be differentiated from ATCC 27449 and other species showing high 16S rRNA gene sequence similarity (98–99 %), as well as other borrelidin-producing strains, based on morphological and physiological characteristics. On the basis of its physiological and molecular properties, it is proposed that strain NEAU-W2 represents a novel species, sp. nov. The type strain is NEAU-W2 ( = CGMCC 4.7004  = ATCC BAA-2424  = DSM 42073).

Funding
This study was supported by the:
  • NKPBR (Award 2010CB126102)
  • NNSF (Award 30771427 and 30971937)
  • Special Foundation for Scientific and Technological Innovation Research of Harbin (Award 2011RFXXN038)
  • Graduate Innovation Scientific Research Project of Heilongjiang Province (Award YJSCX2011-066HLJ)
  • NSF (Award C201029)
  • NIH (Award GM086184)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.041483-0
2013-03-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/1030.html?itemId=/content/journal/ijsem/10.1099/ijs.0.041483-0&mimeType=html&fmt=ahah

References

  1. Collins M. D. ( 1985 ). Isoprenoid quinone analyses in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267284. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  2. Coombs J. T., Franco C. M. M. ( 2003 ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. . Appl Environ Microbiol 69, 56035608. [PubMed] [CrossRef]
    [Google Scholar]
  3. de Araujo J. M., da Silva A. C., Azevedo J. L. ( 2000 ). Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.). . Braz Arch Biol Technol 43, 447451. [CrossRef]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [PubMed] [CrossRef]
    [Google Scholar]
  5. de Oliveira M. F., da Silva M. G., Van Der Sand S. T. ( 2010 ). Anti-phytopathogen potential of endophytic actinobacteria isolated from tomato plants (Lycopersicon esculentum) in southern Brazil, and characterization of Streptomyces sp. R18(6), a potential biocontrol agent. . Res Microbiol 161, 565572. [PubMed] [CrossRef]
    [Google Scholar]
  6. Dorgrrloh M., Kretsehmer A., Schmidt R. R., Steffens R., Zoebelein G., Tietjen K., Roeben W., Stendel W., Saleher O. ( 1988 ). Borreldin insecticide and herbicide and its preparation by fermentation. . Chem Abstr 108, 126704z.
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  8. Goodfellow M., Orchard V. A. ( 1974 ). Antibiotic sensitivity of some nocardioform bacteria and its value as a criterion for taxonomy. . J Gen Microbiol 83, 375387.[PubMed] [CrossRef]
    [Google Scholar]
  9. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Fement Technol 65, 501509. [CrossRef]
    [Google Scholar]
  11. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [PubMed] [CrossRef]
    [Google Scholar]
  12. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 227291. Edited by Dietz A., Thayer J. . Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  13. Lee Y. K., Kim H. W., Liu C. L., Lee H. K. ( 2003 ). A simple method for DNA extraction from marine bacteria that produce extracellular materials. . J Microbiol Methods 52, 245250. [PubMed] [CrossRef]
    [Google Scholar]
  14. Lee S. O., Choi G. J., Choi Y. H., Jang K. S., Park D. J., Kim C. J., Kim J.-C. ( 2008 ). Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae . . J Microbiol Biotechnol 18, 17411746.[PubMed]
    [Google Scholar]
  15. Liu C. X., Zhang J., Wang X. J., Qian P. T., Wang J. D., Gao Y. M., Yan Y. J., Zhang S. Z., Xu P. F. & other authors ( 2012 ). Antifungal activity of borrelidin produced by a Streptomyces strain isolated from soybean. . J Agric Food Chem 60, 12511257. [PubMed] [CrossRef]
    [Google Scholar]
  16. Loqman S., Barka E. A., Clément C., Ouhdouch Y. ( 2009 ). Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. . World J Microbiol Biotechnol 25, 8191. [CrossRef]
    [Google Scholar]
  17. Lyons A. J., Pridham T. G. ( 1971 ). Streptomyces torulosus sp. n., an unusual knobby-spored taxon. . Appl Microbiol 22, 190193.[PubMed]
    [Google Scholar]
  18. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195206.
    [Google Scholar]
  19. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178182. [PubMed] [CrossRef]
    [Google Scholar]
  20. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  22. Nagatsu J., Suzuki S. ( 1963 ). Studies on an antitumor antibiotic cervicarcin. III. Taxonomic studies on the cervicarcin producing organism, Streptomyces ogaensis nov. sp.. J Antibiot (Tokyo) 16, 203206.[PubMed]
    [Google Scholar]
  23. Price K. E., Schmitz H. ( 1966 ). Antibiotic ossamycin and method of preparing same. US Patent 3,271,253.
  24. Saisivam S., Bhikshapathi D. V. R. N., Krishnaveni J., Kishan V. ( 2008 ). Isolation of borrelidin from Streptomyces californicus – an India soil isolate. . Int J Biotechnol 7, 349355.
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Shiang M., Kuo M. Y., Chu K. C., Chang P. C., Chang H. Y., Lee H. P. ( 2001 ). Strain of Streptomyces, and relevant uses thereof. US patent 6,193,964 B1.
  27. Shirling E. B., Gottlieb D. ( 1968 ). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. . Int J Syst Bacteriol 18, 279392. [CrossRef]
    [Google Scholar]
  28. Shirling E. B., Gottlieb D. ( 1969 ). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. . Int J Syst Bacteriol 19, 391512. [CrossRef]
    [Google Scholar]
  29. Singh S. K., Gurusiddaiah S., Whalen J. W. ( 1985 ). Treponemycin, a nitrile antibiotic active against Treponema hyodysenteriae . . Antimicrob Agents Chemother 27, 239245. [PubMed] [CrossRef]
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [PubMed] [CrossRef]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  32. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 17431813.[PubMed]
    [Google Scholar]
  33. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology (English translation of Microbiologiya) 16, 176178.
    [Google Scholar]
  34. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y. ( 2011 ). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). . Int J Syst Evol Microbiol 61, 11651169. [PubMed] [CrossRef]
    [Google Scholar]
  35. Yokota A., Tamura T., Hasegawa T., Huang L. H. ( 1993 ). Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 43, 805812. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.041483-0
Loading
/content/journal/ijsem/10.1099/ijs.0.041483-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error