1887

Abstract

A newly isolated, facultatively methylotrophic bacterium (strain MUSA) was investigated. The isolate was strictly aerobic, Gram-stain-negative, asporogenous, motile, rod-shaped and multiplied by binary fission. The strain utilized methanol, methylamine and an apparently narrow range of multi-carbon compounds, but not methane, dichloromethane or CO/H, as the carbon and energy sources. Growth occurred at pH 5.5–9.5 (optimum, pH 7.0) and 16–40 °C (optimum, 28–30 °C). The major fatty acids of methanol-grown cells were Cω7, C and 11-methyl-Cω7 . The predominant phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. The major ubiquinone was Q-10. The strain had methanol and methylamine dehydrogenases as well as the enzymes of the -methylglutamate pathway (lyases of γ-glutamylmethylamide and -methylglutamate). C assimilation occurs via the isocitrate lyase-negative serine pathway. Ammonium was assimilated by glutamate dehydrogenase and the glutamate cycle (glutamate synthase/glutamine synthetase). The DNA G+C content of the strain was 64.5 mol% (determined from the melting temperature). Based on 16S rRNA gene sequence similarity (97.0–98.9 %) and DNA–DNA relatedness (36–38 %) with representatives of the genus ( IM1 and JZL-4) the isolate was classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MUSA ( = VKM B-2646 = DSM 24986 = CCUG 61696).

Funding
This study was supported by the:
  • Russian Foundation for Basic Research (RFBR) (Award 10-04-00808)
  • RSC (Award 14. 740. 11. 0111)
  • Russian Foundation for Basic Research (RFBR) (Award 10-04-00808)
  • RSC (Award 14. 740. 11. 0111)
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2013-05-01
2024-03-28
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References

  1. Anthony C., Williams P. ( 2003 ). The structure and mechanism of methanol dehydrogenase. . Biochim Biophys Acta 1647, 1823. [View Article] [PubMed]
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  3. Doronina N. V., Braus-Stromeyer S. A., Leisinger T., Trotsenko Y. A. ( 1995 ). Isolation and characterization of a new facultatively methylotrophic bacterium: description of Methylorhabdus multivorans gen. nov., sp. nov.. Syst Appl Microbiol 18, 9298. [View Article]
    [Google Scholar]
  4. Doronina N. V., Trotsenko Y. A., Krausova V. I., Boulygina E. S., Tourova T. P. ( 1998 ). Methylopila capsulata gen. nov., sp. nov., a novel non-pigmented aerobic facultatively methylotrophic bacterium. . Int J Syst Bacteriol 48, 13131321. [View Article] [PubMed]
    [Google Scholar]
  5. Doronina N. V., Trotsenko Y. A., Tourova T. P., Kuznetsov B. B., Leisinger T. ( 2000 ). Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov.–novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane. . Syst Appl Microbiol 23, 210218. [View Article] [PubMed]
    [Google Scholar]
  6. Doronina N. V., Trotsenko Y. A., Tourova T. P., Kuznetsov B. B., Leisinger T. ( 2001 ). Albibacter methylovorans gen. nov., sp. nov., a novel aerobic, facultatively autotrophic and methylotrophic bacterium that utilizes dichloromethane. . Int J Syst Evol Microbiol 51, 10511058. [View Article] [PubMed]
    [Google Scholar]
  7. Fedorov D. N., Doronina N. V., Trotsenko Y. A. ( 2011 ). Phytosymbiosis of aerobic methylobacteria: new facts and views. . Microbiol 80, 443454. (English translation of Mikrobiologiya). [View Article]
    [Google Scholar]
  8. Felsenstein J. ( 1989 ). phylip – phylogeny inference package (version 3.2). . Cladistics 5, 164166.
    [Google Scholar]
  9. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  10. Ivanova E. G., Doronina N. V., Trotsenko Y. A. ( 2007 ). Hansschlegelia plantiphila gen. nov. sp. nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants. . Syst Appl Microbiol 30, 444452. [View Article] [PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  12. Kimura T., Sugahara I., Hanai K., Asahi T. ( 1995 ). Purification and characterization of the new γ-glutamylmethylamide dissimilating enzyme system from Methylophaga sp. AA-30. . Biosci Biotechnol Biochem 59, 648655. [View Article]
    [Google Scholar]
  13. Kolb S. ( 2009 ). Aerobic methanol-oxidizing bacteria in soil. . FEMS Microbiol Lett 300, 110. [View Article] [PubMed]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: John Wiley and Sons;.
    [Google Scholar]
  15. Li L., Zheng J.-W., Hang B.-J., Doronina N. V., Trotsenko Y. A., He J., Li S.-P. ( 2011 ). Methylopila jiangsuensis sp. nov., an aerobic, facultatively methylotrophic bacterium. . Int J Syst Evol Microbiol 61, 15611566. [View Article] [PubMed]
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. ( 1951 ). Protein measurement with the Folin phenol reagent. . J Biol Chem 193, 265275.[PubMed]
    [Google Scholar]
  17. Marmur J. A. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208214. [View Article]
    [Google Scholar]
  18. McDonald I. R., Murrell J. C. ( 1997 ). The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs. . Appl Environ Microbiol 63, 32183224.[PubMed]
    [Google Scholar]
  19. Nash T. ( 1953 ). The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. . Biochem J 55, 416421.[PubMed]
    [Google Scholar]
  20. Neufeld J. D., Schäfer H., Cox M. J., Boden R., McDonald I. R., Murrell J. C. ( 2007 ). Stable-isotope probing implicates Methylophaga spp and novel Gammaproteobacteria in marine methanol and methylamine metabolism. . ISME J 1, 480491. [View Article] [PubMed]
    [Google Scholar]
  21. Owen R. J., Lapage S. P. ( 1976 ). The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. . J Appl Bacteriol 41, 335340. [View Article] [PubMed]
    [Google Scholar]
  22. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  23. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  25. Trotsenko Y. A., Doronina N. V., Govorukhina N. I. ( 1986 ). Metabolism of non-motile obligately methylotrophic bacteria. . FEMS Microbiol Lett 33, 293297. [View Article]
    [Google Scholar]
  26. Van de Peer Y., De Wachter R. ( 1994 ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. . Comput Appl Biosci 10, 569570.[PubMed]
    [Google Scholar]
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