RT Journal Article SR Electronic(1) A1 Shojaei, Hasan A1 Daley, Charles A1 Gitti, Zoe A1 Hashemi, Abodolrazagh A1 Heidarieh, Parvin A1 Moore, Edward R. B. A1 Naser, Abass Daei A1 Russo, Cristina A1 van Ingen, Jakko A1 Tortoli, EnricoYR 2013 T1 Mycobacterium iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_4 SP 1383 OP 1389 DO https://doi.org/10.1099/ijs.0.043562-0 PB Microbiology Society, SN 1466-5034, AB The isolation and characterization of a novel, rapidly growing, scotochromogenic mycobacterial species is reported. Eight independent strains were isolated from clinical specimens from six different countries of the world, two in Iran, two in Italy and one in each of following countries: Greece, the Netherlands, Sweden and the USA. Interestingly, two of the strains were isolated from cerebrospinal fluid. The strains were characterized by rapid growth and presented orange-pigmented scotochromogenic colonies. DNA-based analysis revealed unique sequences in the four regions investigated: the 16S rRNA gene, the rRNA gene internal transcribed spacer 1 and the genes encoding the 65 kDa heat-shock protein and the beta-subunit of RNA polymerase. The phylogenetic analysis placed the strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium gilvum . The genotypic and phenotypic data both strongly supported the inclusion of the strains investigated here as members of a novel species within the genus Mycobacterium; the name Mycobacterium iranicum sp. nov. is proposed to indicate the isolation in Iran of the first recognized strains. The type strain is M05T ( = DSM 45541T = CCUG 62053T = JCM 17461T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.043562-0