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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

sp. nov., an endophytic actinomycete isolated from the coastal halophyte Linn., and emended description of the genus Free

Abstract

A novel actinomycete, designated strain KLBMP 1279, was isolated from surface-sterilized roots of a coastal halophyte, Linn., collected from Jiangsu Province, in the east of China. The taxonomic status of this organism was established using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain KLBMP 1279 was closely related to 42H12-1 (99.5 % 16S rRNA gene sequence similarity), CP153-2 (98.4 %) and AA-826 (97.5 %). Chemotaxonomic characteristics were consistent with its assignment to the genus in that the isolate had -diaminopimelic acid as the diagnostic diamino acid in the cell wall, MK-9(H) as major menaquinone and a polar lipid profile containing diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown aminophospholipids and an unknown phospholipid. The predominant fatty acids were iso-C, iso-C and Cω8. The DNA G+C content was 71.7 mol%. However, DNA–DNA hybridization assays as well as physiological and biochemical analyses differentiated strain KLBMP 1279 from its closest phylogenetic relatives. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, the isolate KLBMP 1279 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is KLBMP 1279 ( = KCTC 19887 = NBRC 108673 = DSM 45764). An emended description of the genus is also proposed.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31101502 and 31000005)
  • Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Award 11KJD210002 and 10KJB180008)
  • Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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2013-06-01
2024-04-25
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References

  1. Cerny G. ( 1978 ). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5, 113–122. [View Article]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  6. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 1129–1133. [View Article] [PubMed]
    [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  8. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J. ( 2005 ). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. . Int J Syst Evol Microbiol 55, 1939–1944. [View Article] [PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  10. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 22–291. Edited by Dietz A., Thayer D. W. . Arlington, VA:: Society for Industrial Microbiology;.
     [Google Scholar]
  11. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57, 1424–1428. [View Article] [PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  13. Mevs U., Stackebrandt E., Schumann P., Gallikowski C. A., Hirsch P. ( 2000 ). Modestobacter multiseptatus gen. nov., sp. nov., a budding actinomycete from soils of the Asgard Range (Transantarctic Mountains). . Int J Syst Evol Microbiol 50, 337–346. [View Article] [PubMed]
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. K. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  15. Qin S., Wang H. B., Chen H. H., Zhang Y. Q., Jiang C. L., Xu L. H., Li W. J. ( 2008 ). Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. . Int J Syst Evol Microbiol 58, 2525–2528. [View Article] [PubMed]
    [Google Scholar]
  16. Qin S., Li J., Chen H. H., Zhao G. Z., Zhu W. Y., Jiang C. L., Xu L. H., Li W. J. ( 2009 ). Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. . Appl Environ Microbiol 75, 6176–6186. [View Article] [PubMed]
    [Google Scholar]
  17. Reddy G. S., Potrafka R. M., Garcia-Pichel F. ( 2007 ). Modestobacter versicolor sp. nov., an actinobacterium from biological soil crusts that produces melanins under oligotrophy, with emended descriptions of the genus Modestobacter and Modestobacter multiseptatus Mevs et al. 2000. . Int J Syst Evol Microbiol 57, 2014–2020. [View Article] [PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 405–425.
     [Google Scholar]
  19. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  20. Schumann P. ( 2011 ). Peptidoglycan Structure. . In Taxonomy of Prokaryotes, Methods in Microbiology, vol. 38, pp. 101–129. Edited by Rainey F., Oren A. . London:: Academic Press;. [View Article]
    [Google Scholar]
  21. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  24. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 1743–1813.[PubMed]
    [Google Scholar]
  25. Xiao J., Luo Y., Xu J., Xie S., Xu J. ( 2011 ). Modestobacter marinus sp. nov., a psychrotolerant actinobacterium from deep-sea sediment, and emended description of the genus Modestobacter . . Int J Syst Evol Microbiol 61, 1710–1714. [View Article] [PubMed]
    [Google Scholar]
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Modestobacter roseus sp. nov., an endophytic actinomycete isolated from the coastal halophyte Salicornia europaea Linn., and emended description of the genus Modestobacter
Int J Syst Evol Microbiol 63, 2197 (2013); https://doi.org/10.1099/ijs.0.044412-0
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