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Volume 63, Issue Pt_5

gen. nov., sp. nov., of the family in the phylum , isolated from Arctic soil Free

Abstract

In the course of a study aimed at isolating bacteria from Arctic soils by a method that selectively enriches for rare bioactive actinomycetes, a Gram-stain-negative, pigmented, non-motile rod, designated MN12-7, was isolated. The salmon-pink strain was, based on 16S rRNA gene sequence similarity, found to be affiliated with the family . Strain MN12-7 was catalase-, oxidase- and cellulase-positive and lacked gelatinase, urease, lipase and pectinase. The predominant cellular fatty acids were summed feature 3 (comprising Cω7 and/or Cω6), iso-C and Cω6. The major respiratory quinone of strain MN12-7 was MK-7, and the major polar lipid was phosphatidylethanolamine in addition to phosphatidylserine, seven unidentified lipids and six unidentified aminolipids. The DNA G+C content of strain MN12-7 was 38 mol%. Strain MN12-7 formed a separate lineage in a cluster containing ‘ comitans’, with which it shared 92.3 % 16S rRNA gene sequence similarity. Based on the phenotypic characteristics and phylogenetic inference, strain MN12-7 is proposed as a representative of a novel species in a new genus, gen. nov., sp. nov. The type strain of the type species is MN12-7 ( = KCTC 32015 = CIP 110422).

  • Published Online:
Funding
This study was supported by the:
  • Council of Scientific and Industrial Research, Government of India
  • NCAOR and Ministry of Earth Sciences, Government of India
© 2013 CSIR
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2013-05-01
2024-04-20
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References

  1. An D.-S., Yin C.-R., Lee S.-T., Cho C.-H. ( 2009 ). Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. . Int J Syst Evol Microbiol 59, 1122–1125. [View Article] [PubMed]
    [Google Scholar]
  2. Asker D., Beppu T., Ueda K. ( 2007 ). Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan. . Appl Microbiol Biotechnol 77, 383–392. [View Article] [PubMed]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 2259–2261. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  5. De Vos P., Truper H. G., Tindall B. J. ( 2005 ). Judicial Commission of the International Committee on Systematics of Prokaryotes; Xth International (IUMS) Congress of Bacteriology and Applied Microbiology; Minutes of the meetings, 28, 29 and 31 July and 1 August 2002, Paris, France. . Int J Syst Evol Microbiol 55, 525–532. [View Article]
    [Google Scholar]
  6. Fautz E., Reichenbach H. ( 1980 ). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8, 87–91. [View Article]
    [Google Scholar]
  7. Gordon N. S., Valenzuela A., Adams S. M., Ramsey P. W., Pollock J. L., Holben W. E., Gannon J. E. ( 2009 ). Pedobacter nyackensis sp. nov., Pedobacter alluvionis sp. nov. and Pedobacter borealis sp. nov., isolated from Montana flood-plain sediment and forest soil. . Int J Syst Evol Microbiol 59, 1720–1726. [View Article] [PubMed]
    [Google Scholar]
  8. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 1129–1133. [View Article] [PubMed]
    [Google Scholar]
  9. He X., Xiao T., Kuang H., Lan X., Tudahong M., Osman G., Fang C., Rahman E. ( 2010 ). Sphingobacterium shayense sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 60, 2377–2381. [View Article] [PubMed]
    [Google Scholar]
  10. Hsu S. C., Lockwood J. L. ( 1975 ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29, 422–426.[PubMed]
    [Google Scholar]
  11. Jacobi C. A., Reichenbach H., Tindall B. J., Stackebrandt E. ( 1996 ). ‘ Candidatus comitans,’ a bacterium living in coculture with Chondromyces crocatus (myxobacteria). . Int J Syst Bacteriol 46, 119–122. [View Article] [PubMed]
    [Google Scholar]
  12. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O. ( 2009a ). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. . Int J Syst Evol Microbiol 59, 1451–1454. [View Article] [PubMed]
    [Google Scholar]
  13. Jeon Y., Kim J. M., Park J. H., Lee S. H., Seong C.-N., Lee S.-S., Jeon C. O. ( 2009b ). Pedobacter oryzae sp. nov., isolated from rice paddy soil. . Int J Syst Evol Microbiol 59, 2491–2495. [View Article] [PubMed]
    [Google Scholar]
  14. Kasana R. C., Salwan R., Dhar H., Dutt S., Gulati A. ( 2008 ). A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine. . Curr Microbiol 57, 503–507. [View Article] [PubMed]
    [Google Scholar]
  15. Kim M. K., Na J. R., Cho D. H., Soung N. K., Yang D. C. ( 2007 ). Parapedobacter koreensis gen. nov., sp. nov.. Int J Syst Evol Microbiol 57, 1336–1341. [View Article] [PubMed]
    [Google Scholar]
  16. Kim S.-J., Weon H.-Y., Kim Y.-S., Yoo S.-H., Kim B.-Y., Anandham R., Kwon S.-W. ( 2010 ). Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost. . Int J Syst Evol Microbiol 60, 1849–1853. [View Article] [PubMed]
    [Google Scholar]
  17. Kobayashi T., Koike K., Yoshimatsu T., Higaki N., Suzumatsu A., Ozawa T., Hatada Y., Ito S. ( 1999 ). Purification and properties of a low-molecular-weight, high-alkaline pectate lyase from an alkaliphilic strain of Bacillus . . Biosci Biotechnol Biochem 63, 65–72. [View Article] [PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  19. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  20. Lee H.-G., Kim S.-G., Im W.-T., Oh H.-M., Lee S.-T. ( 2009 ). Pedobacter composti sp. nov., isolated from compost. . Int J Syst Evol Microbiol 59, 345–349. [View Article] [PubMed]
    [Google Scholar]
  21. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. ( 2009 ). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. . Int J Syst Evol Microbiol 59, 1447–1450. [View Article] [PubMed]
    [Google Scholar]
  22. Luo X., Wang Z., Dai J., Zhang L., Li J., Tang Y., Wang Y., Fang C. ( 2010 ). Pedobacter glucosidilyticus sp. nov., isolated from dry riverbed soil. . Int J Syst Evol Microbiol 60, 229–233. [View Article] [PubMed]
    [Google Scholar]
  23. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  24. Matsuyama H., Katoh H., Ohkushi T., Satoh A., Kawahara K., Yumoto I. ( 2008 ). Sphingobacterium kitahiroshimense sp. nov., isolated from soil. . Int J Syst Evol Microbiol 58, 1576–1579. [View Article] [PubMed]
    [Google Scholar]
  25. Ntougias S., Fasseas C., Zervakis G. I. ( 2007 ). Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete. . Int J Syst Evol Microbiol 57, 398–404. [View Article] [PubMed]
    [Google Scholar]
  26. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. ( 2007 ). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. . Int J Syst Evol Microbiol 57, 2349–2354. [View Article] [PubMed]
    [Google Scholar]
  27. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35, 7188–7196. [View Article] [PubMed]
    [Google Scholar]
  28. Roh S. W., Quan Z.-X., Nam Y.-D., Chang H.-W., Kim K.-H., Kim M.-K., Im W.-T., Jin L., Kim S.-H. & other authors ( 2008 ). Pedobacter agri sp. nov., from soil. . Int J Syst Evol Microbiol 58, 1640–1643. [View Article] [PubMed]
    [Google Scholar]
  29. Shivaji S., Ray M. K., ShyamalaRao N., Saisree L., Jagannadham M. V., Seshu Kumar G., Reddy G. S. N., Bhargava P. M. ( 1992 ). Sphingobacterium antarcticus sp. nov. a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. . Int J Syst Bacteriol 42, 102–106. [View Article]
    [Google Scholar]
  30. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. ( 1986 ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. . J Microbiol Methods 5, 139–156. [View Article]
    [Google Scholar]
  31. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for general and molecular bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, D.C:: American Society for Microbiology;.
     [Google Scholar]
  32. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. ( 1998 ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48, 165–177. [View Article] [PubMed]
    [Google Scholar]
  33. Szabó I., Szoboszlay S., Kriszt B., Háhn J., Harkai P., Baka E., Táncsics A., Kaszab E., Privler Z., Kukolya J. ( 2011 ). Olivibacter oleidegradans sp. nov., a hydrocarbon-degrading bacterium isolated from a biofilter clean-up facility on a hydrocarbon-contaminated site. . Int J Syst Evol Microbiol 61, 2861–2865. [View Article] [PubMed]
    [Google Scholar]
  34. Tamura K., Nei M. ( 1993 ). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. . Mol Biol Evol 10, 512–526.[PubMed]
    [Google Scholar]
  35. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  36. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. ( 2008 ). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. . Int J Syst Evol Microbiol 58, 2046–2050. [View Article] [PubMed]
    [Google Scholar]
  37. Vaz-Moreira I., Nobre M. F., Nunes O. C., Manaia C. M. ( 2007 ). Pseudosphingobacterium domesticum gen. nov., sp. nov., isolated from home-made compost. . Int J Syst Evol Microbiol 57, 1535–1538. [View Article] [PubMed]
    [Google Scholar]
  38. Wei W., Zhou Y., Wang X., Huang X., Lai R. ( 2008 ). Sphingobacteriuman huiense sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 58, 2098–2101. [View Article] [PubMed]
    [Google Scholar]
  39. Weon H.-Y., Kim B.-Y., Lee C.-M., Hong S.-B., Jeon Y.-A., Koo B.-S., Kwon S.-W. ( 2009 ). Solitalea koreensis gen. nov., sp. nov. and the reclassification of [Flexibacter] canadensis as Solitalea canadensis comb. nov.. Int J Syst Evol Microbiol 59, 1969–1975. [View Article] [PubMed]
    [Google Scholar]
  40. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N. ( 1983 ). Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting Gram-negative rods in CDC groups IIK-2 and IIb. . Int J Syst Bacteriol 33, 580–598. [View Article]
    [Google Scholar]
  41. Zhang J., Zheng J.-W., Cho B. C., Hwang C. Y., Fang C., He J., Li S.-P. ( 2012 ). Sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge. . Int J Syst Evol Microbiol 62, 683–687. [View Article] [PubMed]
    [Google Scholar]
  42. Zhao H., Kassama Y., Young M., Kell D. B., Goodacre R. ( 2004 ). Differentiation of Micromonospora isolates from a coastal sediment in Wales on the basis of Fourier transform infrared spectroscopy, 16S rRNA sequence analysis, and the amplified fragment length polymorphism technique. . Appl Environ Microbiol 70, 6619–6627. [View Article] [PubMed]
    [Google Scholar]
  43. Zimmermann J. J., Langer R., Cooney C. L. ( 1990 ). Specific plate assay for bacterial heparinase. . Appl Environ Microbiol 56, 3593–3594.[PubMed]
    [Google Scholar]
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Arcticibacter svalbardensis gen. nov., sp. nov., of the family Sphingobacteriaceae in the phylum Bacteroidetes, isolated from Arctic soil
Int J Syst Evol Microbiol 63, 1627 (2013); https://doi.org/10.1099/ijs.0.044420-0
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