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Volume 63, Issue Pt_7

sp. nov., isolated from Roman catacombs Free

Abstract

A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, strain SC13E-S71, was isolated from tuff, volcanic rock, where the Roman catacombs of Saint Callixtus in Rome, Italy, was excavated. Analysis of 16S rRNA gene sequences revealed that strain SC13E-S71 belongs to the genus , and that it shows the greatest sequence similarity with DSM 14889 (98.72 %), DSM 15583 (98.65 %), LMG 23390 (98.16 %), KCTC 12580 (98.09 %), DSM 13593 (98.09 %), DSM 14551 (98.09 %), DSM 22271 (98.02 %), KCTC 12209 (97.73 %), KACC 10927 (97.49 %), DSM 24316 (97.37 %) and KCTC 22112 (97.09 %). The predominant fatty acids were Cω7, summed feature 3 (iso-C 2-OH and/or Cω7), C 2-OH and C. The predominant menaquinone was MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. These chemotaxonomic data are common to members of the genus . However, a polyphasic approach using physiological tests, DNA base ratios, DNA–DNA hybridization and 16S rRNA gene sequence comparisons showed that the isolate SC13E-S71 belongs to a novel species within the genus , for which the name sp. nov. is proposed. The type strain is SC13E-S71 ( = DSM 25229 = CECT 8016).

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2013-07-01
2024-04-20
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Sphingopyxisitalica sp. nov., isolated from Roman catacombs
Int J Syst Evol Microbiol 63, 2565 (2013); https://doi.org/10.1099/ijs.0.046573-0
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