Bacillushalosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake
Mehrshad, Maliheh and Amoozegar, Mohammad Ali and Didari, Maryam and Bagheri, Maryam and Fazeli, Seyed Abolhassan Shahzadeh and Schumann, Peter and Spröer, Cathrin and Sánchez-Porro, Cristina and Ventosa, Antonio,, 63, 2776-2781 (2013),
doi = https://doi.org/10.1099/ijs.0.046961-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A novel Gram-stain-positive, moderately halophilic bacterium, designated strain E33T, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain E33T were motile rods and produced ellipsoidal endospores at a central or subterminal position in swollen sporangia. Strain E33T was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–25 % (w/v), with optimum growth occurring at 5–15 % (w/v) NaCl. The optimum temperature and pH for growth were 40 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain E33T was shown to belong to the genus Bacillus within the phylum Firmicutes and showed the closest phylogenetic similarity with the species Bacillus niabensis 4T19T (99.2 %), Bacillus herbersteinensis D-1-5aT (97.3 %) and Bacillus litoralis SW-211T (97.2 %). The DNA G+C content of the type strain of the novel species was 42.6 mol%. The major cellular fatty acids of strain E33T were anteiso-C15 : 0 and iso-C15 : 0, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids, an unknown lipid and an unknown phospholipid. The isoprenoid quinones were MK-7 (97 %), MK-6 (2 %) and MK-8 (0.5 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate E33T within the genus Bacillus . DNA–DNA hybridization experiments revealed low levels of relatedness between strain E33T and Bacillus niabensis IBRC-M 10590T (22 %), Bacillus herbersteinensis CCM 7228T (38 %) and Bacillus litoralis DSM 16303T (19 %). On the basis of polyphasic evidence from this study, a novel species of the genus Bacillus , Bacillus halosaccharovorans sp. nov. is proposed, with strain E33T ( = IBRC-M 10095T = DSM 25387T) as the type strain.,
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